AB45027

Anti-MBD2 + MBD3 antibody [106B691]

(1 Review)

(11 Publications)

Mouse Monoclonal MBD2 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human MBD2 aa 200-250.

View Alternative Names

Methyl-CpG-binding domain protein 2, Demethylase, Methyl-CpG-binding protein MBD2, DMTase, MBD2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

IHC-P

AbReview22085****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

ab45027 at 2μg/ml staining MBD2 + MBD3 in human glioma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed and samples were blocked using 10% BSA. The secondary used was an HRP conjugated anti rabbit/mouse mix.

Image courtesy of an anonymous Abreview.

Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Overlay histogram showing HeLa cells stained with ab45027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45027, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Immunohistochemistry analysis of immersion fixed paraffin-embedded sections of human placenta labeling MBD2 + MBD3 with ab45027 at 1/300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Supplier Data

Western blot - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

All lanes:

Western blot - Anti-MBD2 + MBD3 antibody [106B691] (ab45027) at 2 µg/mL

Lane 1:

HeLa cell lysate (nuclear fraction) without immunising peptide

Lane 2:

HeLa cell lysate (nuclear fraction) with immunising peptide

Predicted band size: 45 kDa

Observed band size: 160 kDa,33 kDa,40 kDa,45 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

106B691

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human MBD2 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UBB5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MBD2 and MBD3 are proteins that belong to the methyl-CpG-binding domain (MBD) family. These proteins recognize and bind to methylated DNA aiding in the interpretation of methylation signals in the genome. MBD2 has a molecular mass of approximately 45 kDa while MBD3 is about 34 kDa. These proteins are expressed in various tissues with notable expression in the brain and embryonic tissues. Furthermore both proteins show a conserved domain that allows them to bind to methylated CpG dinucleotides effectively.

Biological function summary

MBD2 and MBD3 participate in gene silencing and chromatin remodeling. They act as part of the NuRD (nucleosome remodeling and deacetylase) complex which integrates both chromatin remodeling and histone deacetylase activities to regulate transcription. Within this complex MBD2 associates with co-repressor proteins and may directly influence transcriptional repression activities. MBD3 although structurally similar lacks strong methyl-CpG binding and may have a more regulatory role in the NuRD complex.

Pathways

MBD2 and MBD3 influence epigenetic regulatory pathways including those involved in gene expression and genomic imprinting. They interact with pathways that involve DNA methylation and histone modification. Within these pathways MBD2 and MBD3 closely work with proteins such as HDAC1 and HDAC2 which are integral to the deacetylation processes in chromatin remodeling. Their roles in these pathways are important for maintaining cellular identity and regulating developmental processes.

MBD2 and MBD3 have associations with cancer and neurological disorders. In cancer MBD2 can contribute to oncogenesis by aberrantly silencing tumor suppressor genes through DNA methylation. Similarly altered expression of MBD3 has been observed in some cancers potentially impacting cell cycle regulation. Regarding neurological disorders dysregulation of MBD2 and MBD3 affects neuronal development and function linking them to developmental disorders like Rett syndrome. These relationships suggest connections with other regulatory proteins involved in epigenetic controls.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (PubMed : 9774669). Binds hemimethylated DNA as well (PubMed : 10947852, PubMed : 24307175). Recruits histone deacetylases and DNA methyltransferases to chromatin (PubMed : 10471499, PubMed : 10947852). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Acts as a transcriptional repressor and plays a role in gene silencing (PubMed : 10471499, PubMed : 10947852, PubMed : 16415179). Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (PubMed : 16415179). May enhance the activation of some unmethylated cAMP-responsive promoters (PubMed : 12665568).

See full target information MBD2

Additional targets

MBD3

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113448 PubMed37737560

2023

BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liao,Yi Wu,Le Qin,Zhiwu Jiang,Shixue Gou,Linfu Zhou,Qilan Hong,Yao Li,Jingxuan Shi,Yao Yao,Liangxue Lai,Yangqiu Li,Pentao Liu,Jean Paul Thiery,Dajiang Qin,Thomas Graf,Xingguo Liu,Peng Li

Genome research 28:1646-1655 PubMed30254051

2018

The gene repressor complex NuRD interacts with the histone variant H3.3 at promoters of active genes.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Kraushaar,Zuozhou Chen,Qingsong Tang,Kairong Cui,Junfang Zhang,Keji Zhao

Cell metabolism 23:881-92 PubMed27166947

2016

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Gómez-Del Arco,Eusebio Perdiguero,Paula Sofia Yunes-Leites,Rebeca Acín-Pérez,Miriam Zeini,Antonio Garcia-Gomez,Krishnamoorthy Sreenivasan,Miguel Jiménez-Alcázar,Jessica Segalés,Dolores López-Maderuelo,Beatriz Ornés,Luis Jesús Jiménez-Borreguero,Gaetano D'Amato,David Enshell-Seijffers,Bruce Morgan,Katia Georgopoulos,Abul B M M K Islam,Thomas Braun,José Luis de la Pompa,Johnny Kim,José A Enriquez,Esteban Ballestar,Pura Muñoz-Cánoves,Juan Miguel Redondo

Oncotarget 7:14476-85 PubMed26910839

2016

MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells.

Applications

Species

Unspecified reactive species

Min Xu,Shihui Bian,Jie Li,Junbo He,Hui Chen,Lu Ge,Zhijun Jiao,Youli Zhang,Wanxin Peng,Fengyi Du,Yinyuan Mo,Aihua Gong

Molecular cell 56:738-48 PubMed25453762

2014

Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Young Kim,Pieter Bas Kwak,Charles J Weitz

International journal of molecular sciences 15:16719-40 PubMed25244019

2014

Sperm and spermatids contain different proteins and bind distinct egg factors.

Applications

Species

Frog

Marta Teperek,Kei Miyamoto,Angela Simeone,Renata Feret,Michael J Deery,John B Gurdon,Jerome Jullien

Cancer research 71:5859-70 PubMed21724586

2011

Overexpression of MBD2 in glioblastoma maintains epigenetic silencing and inhibits the antiangiogenic function of the tumor suppressor gene BAI1.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhu,Stephen B Hunter,Paula M Vertino,Erwin G Van Meir

PloS one 4:e6617 PubMed19672297

2009

Epigenetic differences in cortical neurons from a pair of monozygotic twins discordant for Alzheimer's disease.

Applications

IHC-FoFr

Species

Human

Diego Mastroeni,Ann McKee,Andrew Grover,Joseph Rogers,Paul D Coleman

Neurobiology of aging 31:2025-37 PubMed19117641

2009

Epigenetic changes in Alzheimer's disease: decrements in DNA methylation.

Applications

IHC-Fr

Species

Human

Diego Mastroeni,Andrew Grover,Elaine Delvaux,Charisse Whiteside,Paul D Coleman,Joseph Rogers

Human molecular genetics 14:785-97 PubMed15689352

2005

Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples.

Applications

Unspecified application

Species

Unspecified reactive species

Karen N Thatcher,Sailaja Peddada,Dag H Yasui,Janine M Lasalle

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MBD2 + MBD3 antibody [106B691]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Western blot - Anti-MBD2 + MBD3 antibody [106B691] (AB45027)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (11)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

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