Anti-MBD3 antibody [EPR9913] - BSA and Azide free

5 product images

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  ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

  Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
  The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
  

  Primers and probes are located in the first kb of the transcribed region.
  *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464).

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  ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

  Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
  The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
  

  Primers and probes are located in the first kb of the transcribed region.
  *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464).

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  Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

  Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MBD3 with purified Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).

  Control: PBS only

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464).

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  Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

  Immunofluorescent analysis of HeLa cells labeling MBD3 with Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464 at 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464).

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  Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (ab240144)

  Intracellular flow cytometric analysis of permeabilized 293T cells labeling MBD3 with Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MBD3 antibody [EPR9913] - ChIP Grade ab157464).