AB224809

Anti-MBD4/MED1 antibody

Name: Anti-MBD4/MED1 antibody (ab224809) | Abcam
Brand: Abcam Limited
SKU: AB224809
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal MBD4/MED1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MBD4 aa 1-50.

View Alternative Names

MED1, MBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD4/MED1 antibody (AB224809)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD4/MED1 antibody (AB224809)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MBD4/MED1 using ab224809 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-MBD4/MED1 antibody (AB224809)

MBD4/MED1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224809 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab224809 at 0.1 μg/ml.

Lane 1 : ab224809 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-MBD4/MED1 antibody (ab224809)

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-MBD4/MED1 antibody (AB224809)

All lanes:

Western blot - Anti-MBD4/MED1 antibody (ab224809) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Predicted band size: 66 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MBD4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O95243

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab224809 was affinity purified using an epitope specific to MBD4/MED1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MBD4 protein also known as MED1 is essential in DNA repair and gene regulation. MBD4 contains a methyl-CpG-binding domain allowing it to bind specifically to methylated DNA. The protein has a molecular weight of about 63 kDa. It is expressed in various tissues with higher levels in bone marrow and lymphoid tissues. MBD4 can recognize and excise mismatched T/G base pairs at methylated CpG sites which is important for maintaining genomic stability.

Biological function summary

MBD4 participates in the protection of genome integrity. It is involved in the DNA demethylation process ensuring proper gene expression patterns. MBD4 functions as part of complex DNA repair machinery. By working with other repair proteins MBD4 facilitates the correction of errors arising during DNA replication and demethylation reducing the risk of mutation accumulation and genomic instability.

Pathways

MBD4 plays a significant role in the base excision repair (BER) pathway. This pathway is key for correcting DNA damage caused by deamination and other forms of DNA alteration. In the BER pathway MBD4 cooperates with proteins like DNA glycosylases to identify and remove incorrect bases from the DNA strand. MBD4 also interacts with transcriptional regulation pathways influencing how genes are expressed in response to changes in the epigenetic landscape.

The malfunction of MBD4 links to colorectal cancer and other forms of cancer. Deficiency or mutations in the MBD4 gene increase the likelihood of mutation accumulation leading to tumorigenesis. MBD4's interactions with other proteins involved in DNA repair such as MLH1 highlight its role in maintaining DNA integrity. Genetic instability from impaired MBD4 function could trigger or exacerbate progression of cancerous conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G : T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G : U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

See full target information MBD4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9864 PubMed39543136

2024

Base-excision repair pathway shapes 5-methylcytosine deamination signatures in pan-cancer genomes.

Applications

Unspecified application

Species

Unspecified reactive species

André Bortolini Silveira,Alexandre Houy,Olivier Ganier,Begüm Özemek,Sandra Vanhuele,Anne Vincent-Salomon,Nathalie Cassoux,Pascale Mariani,Gaelle Pierron,Serge Leyvraz,Damian Rieke,Alberto Picca,Franck Bielle,Marie-Laure Yaspo,Manuel Rodrigues,Marc-Henri Stern

Nephrology (Carlton, Vic.) 29:555-564 PubMed39011853

2024

Rhodojaponin VI ameliorates podocyte injury related with MDM2/Notch1 pathway in rat experimental membranous nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Anni Song,Ruiwei Yan,Yue Qiu,Xingjie Yin,Jing Xiong,Guangmin Yao,Chun Zhang

Molecular cell 84:2036-2052.e7 PubMed38688279

2024

Unprocessed genomic uracil as a source of DNA replication stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Christopher S Nabel,Turner W Seay,Parasvi S Patel,Ajinkya S Kawale,Caroline R Crosby,Helene Tigro,Eugene Oh,Matthew G Vander Heiden,Aaron N Hata,Zucai Suo,Lee Zou

NPJ precision oncology 6:81 PubMed36323843

2022

Cytidine analogs are synthetic lethal with base excision repair default due to MBD4 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Chabot,Fariba Nemati,Aurélie Herbette,Alexandre Demeyer,Stéphane Dayot,Olivier Ganier,Samar Alsafadi,Sophie Gardrat,Pascale Mariani,Marie Luporsi,Maxime Corbé,Vincent Servois,Nathalie Cassoux,Didier Decaudin,Sergio Roman Roman,Elaine Del Nery,Sophie Piperno-Neumann,Marc-Henri Stern,Manuel Rodrigues

Nature 606:930-936 PubMed35477155

2022

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shangming Tang,Ema Stokasimov,Yuxiang Cui,David Pellman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MBD4/MED1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD4/MED1 antibody (AB224809)

Immunoprecipitation - Anti-MBD4/MED1 antibody (AB224809)

Western blot - Anti-MBD4/MED1 antibody (AB224809)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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