AB191037

Anti-MBD4/MED1 antibody - C-terminal

Name: Anti-MBD4/MED1 antibody - C-terminal (ab191037) | Abcam
Brand: Abcam Limited
SKU: AB191037
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MBD4/MED1 antibody. C-terminal. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MBD4 aa 550 to C-terminus.

View Alternative Names

MED1, MBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase

3 Images

Supplier Data

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

E.coli derived -recombinant Human MBD4/MED1, 39.7 kDa (162aa tag + Q400-S580).

All lanes:

Western blot - Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/mL

Lane 1:

Recombinant Human MBD4/MED1 protein (aa400-580) at 0.01 µg

Lane 2:

Recombinant Human MBD4/MED1 protein (aa400-580) at 0.005 µg

Lane 3:

Recombinant Human MBD4/MED1 protein (aa400-580) at 0.0025 µg

Predicted band size: 66 kDa

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Supplier Data

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

All lanes:

Western blot - Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysate

Lane 2:

Rat kidney tissue lysate

Lane 3:

A549 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

MCF-7 cell lysate

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

All lanes:

Western blot - Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysate

Lane 2:

MCF-7 cell lysate

Lane 3:

HeLa cell lysate

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human MBD4 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95243

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for MBD4 is approximately 1ng/lane under reducing conditions.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for MBD4 is approximately 1ng/lane under reducing conditions.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MBD4 protein also known as MED1 is essential in DNA repair and gene regulation. MBD4 contains a methyl-CpG-binding domain allowing it to bind specifically to methylated DNA. The protein has a molecular weight of about 63 kDa. It is expressed in various tissues with higher levels in bone marrow and lymphoid tissues. MBD4 can recognize and excise mismatched T/G base pairs at methylated CpG sites which is important for maintaining genomic stability.

Biological function summary

MBD4 participates in the protection of genome integrity. It is involved in the DNA demethylation process ensuring proper gene expression patterns. MBD4 functions as part of complex DNA repair machinery. By working with other repair proteins MBD4 facilitates the correction of errors arising during DNA replication and demethylation reducing the risk of mutation accumulation and genomic instability.

Pathways

MBD4 plays a significant role in the base excision repair (BER) pathway. This pathway is key for correcting DNA damage caused by deamination and other forms of DNA alteration. In the BER pathway MBD4 cooperates with proteins like DNA glycosylases to identify and remove incorrect bases from the DNA strand. MBD4 also interacts with transcriptional regulation pathways influencing how genes are expressed in response to changes in the epigenetic landscape.

The malfunction of MBD4 links to colorectal cancer and other forms of cancer. Deficiency or mutations in the MBD4 gene increase the likelihood of mutation accumulation leading to tumorigenesis. MBD4's interactions with other proteins involved in DNA repair such as MLH1 highlight its role in maintaining DNA integrity. Genetic instability from impaired MBD4 function could trigger or exacerbate progression of cancerous conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G : T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G : U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

See full target information MBD4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e39861 PubMed39605834

2024

Pan-cancer analysis of IRF1 focusing on prognostic and immunological roles in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiling Sun,Kui Cao,Siran Wang,Mengdi Lu,Jianqun Ma,Chunlong Wu,Yanbin Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MBD4/MED1 antibody - C-terminal

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

Western blot - Anti-MBD4/MED1 antibody - C-terminal (AB191037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise