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Rabbit Polyclonal MBD6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MBD6 aa 600-750.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD6 antibody (AB204403), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MBD6 antibody (AB204403), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MBD6 aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DN6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:24634419). Important for stability of PR-DUB components and stimulating its ubiquitinase activity (PubMed:36180891). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways (PubMed:36180891). Heterochromatin recruitment is not mediated by DNA methylation (PubMed:20700456). The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability; may promote cancer cell growth (PubMed:36180891).

Alternative names

Recommended products

Rabbit Polyclonal MBD6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MBD6 aa 600-750.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MBD6 aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DN6
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MBD6 also known as methyl-CpG-binding domain protein 6 acts by binding to methylated DNA but the exact mechanistic role in transcription regulation remains not fully defined. It has an approximate molecular weight of 131 kDa. Researchers have observed MBD6 expression mainly in the nucleus of cells where it likely interacts with other nuclear proteins. It may play a role in chromatin organization due to its DNA-binding properties.

Biological function summary

MBD6 influences gene expression through its association with chromatin modification processes. It often functions as part of a protein complex involved in reading DNA methylation marks which affects transcriptional regulation. This complex includes proteins engaged in methylation-dependent mechanisms to modify chromatin structure and modulate gene expression.

Pathways

The role of MBD6 integrates within DNA methylation and chromatin remodeling pathways. These pathways are essential for regulating cell differentiation and development. MBD6 interacts with proteins such as MeCP2 and MBD2 which also participate in chromatin remodeling and transcriptional repression by reading methylation signals and executing change in gene expression profiles.

Associated diseases and disorders

Scientists have linked MBD6 to neurological disorders and certain cancer types. It shares pathways with MeCP2 which associates with Rett syndrome implicating potential roles of MBD6 in neural development and function. Additionally MBD6's involvement in chromatin modulation connects it to the dysregulation observed in some cancers suggesting its influence on cancer progression may be due to altered methylation and gene expression profiles.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD6 antibody (ab204403), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD6 antibody (ab204403)

    Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling MBD6 with ab204403 at 1/50 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD6 antibody (ab204403), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MBD6 antibody (ab204403)

    Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling MBD6 with ab204403 at 4 μg/mL (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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