Rabbit Polyclonal MBD6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MBD6 aa 600-750.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:24634419). Important for stability of PR-DUB components and stimulating its ubiquitinase activity (PubMed:36180891). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways (PubMed:36180891). Heterochromatin recruitment is not mediated by DNA methylation (PubMed:20700456). The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability; may promote cancer cell growth (PubMed:36180891).
KIAA1887, MBD6, Methyl-CpG-binding domain protein 6, Methyl-CpG-binding protein MBD6
Rabbit Polyclonal MBD6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MBD6 aa 600-750.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
MBD6 also known as methyl-CpG-binding domain protein 6 acts by binding to methylated DNA but the exact mechanistic role in transcription regulation remains not fully defined. It has an approximate molecular weight of 131 kDa. Researchers have observed MBD6 expression mainly in the nucleus of cells where it likely interacts with other nuclear proteins. It may play a role in chromatin organization due to its DNA-binding properties.
MBD6 influences gene expression through its association with chromatin modification processes. It often functions as part of a protein complex involved in reading DNA methylation marks which affects transcriptional regulation. This complex includes proteins engaged in methylation-dependent mechanisms to modify chromatin structure and modulate gene expression.
The role of MBD6 integrates within DNA methylation and chromatin remodeling pathways. These pathways are essential for regulating cell differentiation and development. MBD6 interacts with proteins such as MeCP2 and MBD2 which also participate in chromatin remodeling and transcriptional repression by reading methylation signals and executing change in gene expression profiles.
Scientists have linked MBD6 to neurological disorders and certain cancer types. It shares pathways with MeCP2 which associates with Rett syndrome implicating potential roles of MBD6 in neural development and function. Additionally MBD6's involvement in chromatin modulation connects it to the dysregulation observed in some cancers suggesting its influence on cancer progression may be due to altered methylation and gene expression profiles.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling MBD6 with ab204403 at 1/50 dilution.
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling MBD6 with ab204403 at 4 μg/mL (green).
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