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AB204403

Anti-MBD6 antibody

0

(1 Review)

|

(2 Publications)

Rabbit Polyclonal MBD6 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MBD6 aa 600-750.

View Alternative Names

KIAA1887, MBD6, Methyl-CpG-binding domain protein 6, Methyl-CpG-binding protein MBD6

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD6 antibody (AB204403)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD6 antibody (AB204403)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling MBD6 with ab204403 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MBD6 antibody (AB204403)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MBD6 antibody (AB204403)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling MBD6 with ab204403 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MBD6 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

Q96DN6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MBD6 also known as methyl-CpG-binding domain protein 6 acts by binding to methylated DNA but the exact mechanistic role in transcription regulation remains not fully defined. It has an approximate molecular weight of 131 kDa. Researchers have observed MBD6 expression mainly in the nucleus of cells where it likely interacts with other nuclear proteins. It may play a role in chromatin organization due to its DNA-binding properties.
Biological function summary

MBD6 influences gene expression through its association with chromatin modification processes. It often functions as part of a protein complex involved in reading DNA methylation marks which affects transcriptional regulation. This complex includes proteins engaged in methylation-dependent mechanisms to modify chromatin structure and modulate gene expression.

Pathways

The role of MBD6 integrates within DNA methylation and chromatin remodeling pathways. These pathways are essential for regulating cell differentiation and development. MBD6 interacts with proteins such as MeCP2 and MBD2 which also participate in chromatin remodeling and transcriptional repression by reading methylation signals and executing change in gene expression profiles.

Scientists have linked MBD6 to neurological disorders and certain cancer types. It shares pathways with MeCP2 which associates with Rett syndrome implicating potential roles of MBD6 in neural development and function. Additionally MBD6's involvement in chromatin modulation connects it to the dysregulation observed in some cancers suggesting its influence on cancer progression may be due to altered methylation and gene expression profiles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed : 24634419). Important for stability of PR-DUB components and stimulating its ubiquitinase activity (PubMed : 36180891). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed : 36180891). MBD5 and MBD6 containing complexes associate with distinct chromatin regions enriched in genes involved in different pathways (PubMed : 36180891). Heterochromatin recruitment is not mediated by DNA methylation (PubMed : 20700456). The PR-DUB complex is an epigenetic regulator of gene expression, including genes involved in development, cell communication, signaling, cell proliferation and cell viability; may promote cancer cell growth (PubMed : 36180891).
See full target information MBD6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

AIDS (London, England) 39:229-240 PubMed39612239

2024

Functional variability of Nef in antagonizing SERINC5 during acute to chronic HIV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Weiting Li,Guoqing Li,Yuyang Liu,Lina Meng,Tianxin Zhang,Libian Wang,Haochen Li,Bin Yu,Jiaxin Wu,Chu Wang,Xianghui Yu

Nature communications 12:3428 PubMed34103526

2021

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hui Wang,Sha Xu,Xiang-Yu Zhou,Rui Zhao,Yan Lin,Jing Cao,Wei-Dong Zang,Hui Tao,Wei Xu,Ming-Qing Li,Shi-Min Zhao,Li-Ping Jin,Jian-Yuan Zhao
View all publications
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Product promise

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