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AB122411

Anti-MBLAC2 antibody

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(2 Publications)

Rabbit Polyclonal MBLAC2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MBLAC2 aa 100-200.

View Alternative Names

Acyl-coenzyme A thioesterase MBLAC2, Acyl-CoA thioesterase MBLAC2, Beta-lactamase MBLAC2, Metallo-beta-lactamase domain-containing protein 2, Palmitoyl-coenzyme A thioesterase MBLAC2, MBLAC2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBLAC2 antibody (AB122411)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBLAC2 antibody (AB122411)

ab122411 at 1/50 dilution staining MBLAC2 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-MBLAC2 antibody (AB122411)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MBLAC2 antibody (AB122411)

Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling MBLAC2 with ab122411 shows positive staining in the intermediate filaments.

Western blot - Anti-MBLAC2 antibody (AB122411)
  • WB

Unknown

Western blot - Anti-MBLAC2 antibody (AB122411)

10-20% SDS PAGE

All lanes:

Western blot - Anti-MBLAC2 antibody (ab122411) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human Plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MBLAC2 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q68D91

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBL1+MBL2 antibody [EPR18381-156] (AB190834)

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MBLAC2 also known as Metallo-beta-lactamase domain-containing protein 2 belongs to the metalloproteinase family. This protein has a molecular mass of around 26 kDa. MBLAC2 is expressed in various tissues including the liver and kidney. Its mechanistic role involves the hydrolysis of certain substrates through the action of its metal-ion-dependent enzymatic domain.
Biological function summary

MBLAC2 participates in cellular processes that require metalloprotease activity. Although not extensively characterized MBLAC2 contributes to the turnover of nucleic acids or proteins given its enzymatic capabilities. MBLAC2's role as a standalone protein suggests it does not form part of significant heteromeric protein complexes but it might still interact transiently with other cellular components involved in RNA or protein metabolism.

Pathways

MBLAC2 interacts mainly within pathways that involve RNA regulation and degradation. It has potential connections to RNA salvage pathways and may interact with proteins like Dicer known to participate in RNA processing. This relation suggests a link to gene expression regulation by influencing the availability of RNA substrates.

MBLAC2 exhibits associations with specific conditions such as certain cancers and neurodegenerative disorders. Its involvement in cancer could relate to its role in RNA metabolism potentially influencing tumorigenesis. Connections to proteins like BRCA1 in cancer pathways might highlight its relevance to DNA repair and genomic stability therefore linking its malfunction to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed : 33219126). Has an acyl-CoA thioesterase activity towards the long chain fatty acyl-CoA thioester palmitoyl-CoA (hexadecanoyl-CoA; C16 : 0-CoA) (PubMed : 33219126). Displays a substrate preference for fatty acyl-CoAs with chain-lengths C12-C18 (PubMed : 33219126). Possesses beta-lactamase activity, catalyzing the hydrolysis of penicillin G and nitrocefin (PubMed : 31434986). Exhibits no activity towards other beta-lactam antibiotic classes including cephalosporins (cefotaxime) and carbapenems (imipenem) (PubMed : 31434986).
See full target information MBLAC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:9397-9414 PubMed37526268

2023

The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew John Eastham,Andria Pelava,Graeme Raymond Wells,Justine Katherine Lee,Isabella Rachel Lawrence,Joshua Stewart,Maria Deichner,Regina Hertle,Nicholas James Watkins,Claudia Schneider

Nature chemical biology 18:812-820 PubMed35484434

2022

Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target.

Applications

Unspecified application

Species

Unspecified reactive species

Severin Lechner,Martin Ian P Malgapo,Christian Grätz,Raphael R Steimbach,Agnes Baron,Patrick Rüther,Simon Nadal,Carmen Stumpf,Christina Loos,Xin Ku,Polina Prokofeva,Ludwig Lautenbacher,Tino Heimburg,Vivian Würf,Chen Meng,Mathias Wilhelm,Wolfgang Sippl,Karin Kleigrewe,Josch K Pauling,Karl Kramer,Aubry K Miller,Michael W Pfaffl,Maurine E Linder,Bernhard Kuster,Guillaume Médard
View all publications
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Product promise

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