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AB77017

Anti-MBNL1 antibody [1D11]

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(4 Publications)

Mouse Monoclonal MBNL1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MBNL1.

View Alternative Names

EXP, KIAA0428, MBNL, MBNL1, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein

2 Images
Western blot - Anti-MBNL1 antibody [1D11] (AB77017)
  • WB

Unknown

Western blot - Anti-MBNL1 antibody [1D11] (AB77017)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MBNL1 antibody [1D11] (ab77017) at 1/500 dilution

All lanes:

HeLa cell lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 42 kDa

Observed band size: 41 kDa

false

Western blot - Anti-MBNL1 antibody [1D11] (AB77017)
  • WB

Unknown

Western blot - Anti-MBNL1 antibody [1D11] (AB77017)

Molecular weight of the tag alone is 26 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MBNL1 antibody [1D11] (ab77017) at 5 µg/mL

All lanes:

recombinant tagged full length human MBNL1 at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Predicted band size: 42 kDa

Observed band size: 64 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1D11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MBNL1.

Q9NR56

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 30th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MBNL1 protein also known as Muscleblind-like protein 1 or MBNL1 is a RNA-binding protein with a molecular mass of approximately 38 kDa. This protein has a central role in the regulation of alternative splicing. It binds to specific RNA motifs and affects the splicing of pre-mRNA. MBNL1 is expressed widely in many tissues including skeletal and cardiac muscles as well as the brain indicating its wide-ranging importance in various cellular processes.
Biological function summary

The MBNL1 protein is involved in the regulation of mRNA metabolism. It functions not just individually but often as a part of larger ribonucleic complexes impacting RNA stability and translation. MBNL1 controls the alternative splicing of numerous transcripts which is necessary for the proper development and function of tissues. Disruptions in MBNL1 activity can lead to mis-splicing of transcripts which demonstrates the key role it plays in maintaining cellular homeostasis.

Pathways

The MBNL1 protein contributes significantly to splicing regulation and muscle-specific gene expression pathways. It interacts with proteins like CELF1 which modulate pre-mRNA processing. These pathways are vital for muscle cell differentiation and maintenance with MBNL1 acting alongside other splicing factors to ensure the correct expression patterns necessary for tissue-specific functions. The balance between MBNL1 and its interacting partners influences splicing outcomes and gene expression.

The dysregulation of MBNL1 activity is implicated in myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2). In these conditions expanded CUG or CCUG repeat RNAs sequester MBNL1 disrupting its function and leading to splicing abnormalities. Through this mechanism MBNL1 connects to other proteins like CUGBP1 which also influences splicing in muscle and other tissues. The resulting mis-splicing from impaired MBNL1 activity underlies many symptoms seen in these muscular disorders highlighting the protein's clinical relevance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events (PubMed : 37548402). Regulates NCOR2 alternative splicing (By similarity).
See full target information MBNL1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 34:102024 PubMed37744174

2023

Peptide-conjugated antimiRs improve myotonic dystrophy type 1 phenotypes by promoting endogenous MBNL1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Irene González-Martínez,Estefanía Cerro-Herreros,Nerea Moreno,Andrea García-Rey,Jorge Espinosa-Espinosa,Marc Carrascosa-Sàez,Diego Piqueras-Losilla,Andrey Arzumanov,David Seoane-Miraz,Yahya Jad,Richard Raz,Matthew J Wood,Miguel A Varela,Beatriz Llamusí,Rubén Artero

Methods in molecular biology (Clifton, N.J.) 2434:207-215 PubMed35213019

2022

Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for the Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Moreno,Irene González-Martínez,Rubén Artero,Estefanía Cerro-Herreros

Scientific reports 11:19417 PubMed34593893

2021

Defined D-hexapeptides bind CUG repeats and rescue phenotypes of myotonic dystrophy myotubes in a Drosophila model of the disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rapisarda,Ariadna Bargiela,Beatriz Llamusi,Isabel Pont,Roger Estrada-Tejedor,Enrique Garcia-España,Ruben Artero,Manuel Perez-Alonso

Nature communications 9:2482 PubMed29946070

2018

miR-23b and miR-218 silencing increase Muscleblind-like expression and alleviate myotonic dystrophy phenotypes in mammalian models.

Applications

Unspecified application

Species

Unspecified reactive species

Estefania Cerro-Herreros,Maria Sabater-Arcis,Juan M Fernandez-Costa,Nerea Moreno,Manuel Perez-Alonso,Beatriz Llamusi,Ruben Artero
View all publications
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