Anti-MBNL1 antibody (ab45899) is a rabbit polyclonal antibody detecting MBNL1 in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 10 publications
- Trusted since 2009
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- Nature communications 14:19562023Correction of Clcn1 alternative splicing reverses muscle fiber type transition in mice with myotonic dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNingyan Hu,Eunjoo Kim,Layal Antoury,Thurman M WheelerPubMed 37029100
- Pharmaceutics 15:2023BlockmiR AONs as Site-Specific Therapeutic MBNL Modulation in Myotonic Dystrophy 2D and 3D Muscle Cells and HSA Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah J Overby,Estefanía Cerro-Herreros,Jorge Espinosa-Espinosa,Irene González-Martínez,Nerea Moreno,Juan M Fernández-Costa,Jordina Balaguer-Trias,Javier Ramón-Azcón,Manuel Pérez-Alonso,Thorleif Møller,Beatriz Llamusí,Rubén ArteroPubMed 37111604
- iScience 26:1065022023Network model integrated with multi-omic data predicts MBNL1 signals that drive myofibroblast activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnders R Nelson,Darrian Bugg,Jennifer Davis,Jeffrey J SaucermanPubMed 37091233
- Life (Basel, Switzerland) 13:2022RNA Foci Formation in a Retinal Glial Model for Spinocerebellar Ataxia Type 7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRocío Suárez-Sánchez,Rodolfo Daniel Ávila-Avilés,J Manuel Hernández-Hernández,Daniel Sánchez-Celis,Cuauhtli N Azotla-Vilchis,Enue R Gómez-Macías,Norberto Leyva-García,Arturo Ortega,Jonathan J Magaña,Bulmaro Cisneros,Oscar Hernández-HernándezPubMed 36675972
- JCI insight 7:2022Senescence plays a role in myotonic dystrophy type 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikel García-Puga,Ander Saenz-Antoñanzas,Gorka Gerenu,Alex Arrieta-Legorburu,Roberto Fernández-Torrón,Miren Zulaica,Amets Saenz,Joseba Elizazu,Gisela Nogales-Gadea,Shahinaz M Gadalla,Marcos J Araúzo-Bravo,Adolfo López de Munain,Ander MatheuPubMed 36040809
- Annals of neurology 92:512-5262022GGC Repeat Expansion of RILPL1 is Associated with Oculopharyngodistal Myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Heng Zeng,Kang Yang,Gan-Qin Du,Yi-Kun Chen,Chun-Yan Cao,Yu-Sen Qiu,Jin He,Hai-Dong Lv,Qian-Qian Qu,Jian-Nan Chen,Guo-Rong Xu,Long Chen,Fu-Ze Zheng,Miao Zhao,Min-Ting Lin,Wan-Jin Chen,Jing Hu,Zhi-Qiang Wang,Ning WangPubMed 35700120
- Cell stem cell 29:419-433.e102022MBNL1 drives dynamic transitions between fibroblasts and myofibroblasts in cardiac wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarrian Bugg,Logan R J Bailey,Ross C Bretherton,Kylie E Beach,Isabella M Reichardt,Kalen Z Robeson,Anna C Reese,Jagadambika Gunaje,Galina Flint,Cole A DeForest,April Stempien-Otero,Jennifer DavisPubMed 35176223
- Neuron 110:1173-1192.e72022Uninterrupted CAG repeat drives striatum-selective transcriptionopathy and nuclear pathogenesis in human Huntingtin BAC mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofeng Gu,Jeffrey Richman,Peter Langfelder,Nan Wang,Shasha Zhang,Monica Bañez-Coronel,Huei-Bin Wang,Lucia Yang,Lalini Ramanathan,Linna Deng,Chang Sin Park,Christopher R Choi,Jeffrey P Cantle,Fuying Gao,Michelle Gray,Giovanni Coppola,Gillian P Bates,Laura P W Ranum,Steve Horvath,Christopher S Colwell,X William YangPubMed 35114102
- Molecular therapy : the journal of the American Society of Gene Therapy 30:75-912021Comprehensive transcriptome-wide analysis of spliceopathy correction of myotonic dystrophy using CRISPR-Cas9 in iPSCs-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumitava Dastidar,Debanjana Majumdar,Jaitip Tipanee,Kshitiz Singh,Arnaud F Klein,Denis Furling,Marinee K Chuah,Thierry VandenDriesschePubMed 34371182
- Biomolecules 11:2021Transcriptome Analysis Reveals Altered Inflammatory Pathway in an Inducible Glial Cell Model of Myotonic Dystrophy Type 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuauhtli N Azotla-Vilchis et. al.PubMed 33530452
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events (PubMed:37548402). Regulates NCOR2 alternative splicing (By similarity).
Alternative names
EXP, KIAA0428, MBNL, MBNL1, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein
Recommended products
Anti-MBNL1 antibody (ab45899) is a rabbit polyclonal antibody detecting MBNL1 in Western Blot, IHC-P, ICC/IF. Suitable for Human.
- Over 10 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MBNL1 antibody (ab45899) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of MBNL1?
Anti-MBNL1 (ab45899) specifically detects a band for MBNL1 (UniProt: Q9NR56) at a molecular weight of 35,40,42kDa.
Trusted by the scientific community
Anti-MBNL1 (ab45899) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MBNL1 protein also known as Muscleblind-like protein 1 or MBNL1 is a RNA-binding protein with a molecular mass of approximately 38 kDa. This protein has a central role in the regulation of alternative splicing. It binds to specific RNA motifs and affects the splicing of pre-mRNA. MBNL1 is expressed widely in many tissues including skeletal and cardiac muscles as well as the brain indicating its wide-ranging importance in various cellular processes.
- Biological function summary
The MBNL1 protein is involved in the regulation of mRNA metabolism. It functions not just individually but often as a part of larger ribonucleic complexes impacting RNA stability and translation. MBNL1 controls the alternative splicing of numerous transcripts which is necessary for the proper development and function of tissues. Disruptions in MBNL1 activity can lead to mis-splicing of transcripts which demonstrates the key role it plays in maintaining cellular homeostasis.
- Pathways
The MBNL1 protein contributes significantly to splicing regulation and muscle-specific gene expression pathways. It interacts with proteins like CELF1 which modulate pre-mRNA processing. These pathways are vital for muscle cell differentiation and maintenance with MBNL1 acting alongside other splicing factors to ensure the correct expression patterns necessary for tissue-specific functions. The balance between MBNL1 and its interacting partners influences splicing outcomes and gene expression.
- Associated diseases and disorders
The dysregulation of MBNL1 activity is implicated in myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2). In these conditions expanded CUG or CCUG repeat RNAs sequester MBNL1 disrupting its function and leading to splicing abnormalities. Through this mechanism MBNL1 connects to other proteins like CUGBP1 which also influences splicing in muscle and other tissues. The resulting mis-splicing from impaired MBNL1 activity underlies many symptoms seen in these muscular disorders highlighting the protein's clinical relevance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-MBNL1 antibody (ab45899)
ab45899 recognises all three known isoforms to MBNL1
All lanes: Western blot - Anti-MBNL1 antibody (ab45899) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat nuclear extract lysate (ab14844) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 110 kDa, 35 kDa, 40 kDa, 42 kDa, 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBNL1 antibody (ab45899)
IHC image of ab45899 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45899, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody (ab45899)
ab45899 staining MBNL1 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab45899 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-MBNL1 antibody (ab45899)
MBNL1 western blot using anti-MBNL1 antibody ab45899. Publication image and figure legend from García-Puga, M., Saenz-Antoñanzas, A., et al., 2020, Aging (Albany NY), PubMed 32310829.
ab45899 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab45899 please see the product overview.
DM1-derived fibroblasts present impaired metabolism. (A) Kinetic normalized OCR response in DM1 and control fibroblasts in basal conditions and after consecutive addition of Oligomycin 1.5 μM, FCCP 1.5 μM and Antimycin-A/Rotenone 1.5 μM. A representative experiment out of 3 is shown with 3 independent control cultures and 2 DM1. (B, C) Quantification of mitochondrial respiratory functions and coupling efficiency in DM1 (n=7) and control fibroblasts (n=3). (D) Representative energy map and (E) Quantification of metabolic potential of DM1 and control fibroblasts. Stressed indicates the values of OCR and ECAR after the injection of oligomycin and FCCP simultaneously. Results are obtained from controls (n=3) and DM1 (n=5) cultures. (F) Representative immunoblots of phospho-AKT, AKT, DMPK and MBNL1 in DM1-derived fibroblasts and healthy controls (n=3).
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