Rabbit Recombinant Monoclonal MBNL1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Transfected cell lysate samples.
Images
![Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--flow-cytometry-intracellular-img418708.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349)")
![Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--flow-cytometry-intracellular-img418707.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349)")
![Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunocytochemistry-immunofluorescence-img418697.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349)")
![Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunocytochemistry-immunofluorescence-img418696.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349)")
![Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunoprecipitation-img418675.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (AB309349)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IP | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Transfected cell lysate | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Transfected cell lysate | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Transfected cell lysate | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Transfected cell lysate | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events (PubMed:37548402). Regulates NCOR2 alternative splicing (By similarity).
Alternative names
EXP, KIAA0428, MBNL, MBNL1, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein
Recommended products
Rabbit Recombinant Monoclonal MBNL1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Transfected cell lysate samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26983-11
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MBNL1 protein also known as Muscleblind-like protein 1 or MBNL1 is a RNA-binding protein with a molecular mass of approximately 38 kDa. This protein has a central role in the regulation of alternative splicing. It binds to specific RNA motifs and affects the splicing of pre-mRNA. MBNL1 is expressed widely in many tissues including skeletal and cardiac muscles as well as the brain indicating its wide-ranging importance in various cellular processes.
- Biological function summary
The MBNL1 protein is involved in the regulation of mRNA metabolism. It functions not just individually but often as a part of larger ribonucleic complexes impacting RNA stability and translation. MBNL1 controls the alternative splicing of numerous transcripts which is necessary for the proper development and function of tissues. Disruptions in MBNL1 activity can lead to mis-splicing of transcripts which demonstrates the key role it plays in maintaining cellular homeostasis.
- Pathways
The MBNL1 protein contributes significantly to splicing regulation and muscle-specific gene expression pathways. It interacts with proteins like CELF1 which modulate pre-mRNA processing. These pathways are vital for muscle cell differentiation and maintenance with MBNL1 acting alongside other splicing factors to ensure the correct expression patterns necessary for tissue-specific functions. The balance between MBNL1 and its interacting partners influences splicing outcomes and gene expression.
- Associated diseases and disorders
The dysregulation of MBNL1 activity is implicated in myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2). In these conditions expanded CUG or CCUG repeat RNAs sequester MBNL1 disrupting its function and leading to splicing abnormalities. Through this mechanism MBNL1 connects to other proteins like CUGBP1 which also influences splicing in muscle and other tissues. The resulting mis-splicing from impaired MBNL1 activity underlies many symptoms seen in these muscular disorders highlighting the protein's clinical relevance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--flow-cytometry-intracellular-img418708.jpg "Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling MBNL1 with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--flow-cytometry-intracellular-img418707.jpg "Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Flow Cytometry (Intracellular) - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling MBNL1 with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunocytochemistry-immunofluorescence-img418697.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblasts) cells labelling MBNL1 with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/500 (0.976 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in C2C12 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunocytochemistry-immunofluorescence-img418696.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Immunocytochemistry/ Immunofluorescence - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labelling MBNL1 with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/500 (0.976 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in Jurkat cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.![Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunoprecipitation-img418675.jpg "Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
MBNL1 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 (mouse myoblast) whole cell lysate 10 ug
Lane 2: C2C12 (mouse myoblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MBNL1 antibody [EPR26983-11] ab309348 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.All lanes: Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] (Anti-MBNL1 antibody [EPR26983-11] ab309348) at 1/30 dilution
All lanes: C2C12 (mouse myoblast) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
![Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunoprecipitation-img418674.jpg "Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
MBNL1 was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate with Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MBNL1 antibody [EPR26983-11] ab309348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate 10 ug
Lane 2: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MBNL1 antibody [EPR26983-11] ab309348 in Jurkat whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 secondsAll lanes: Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] (Anti-MBNL1 antibody [EPR26983-11] ab309348) at 1/30 dilution
All lanes: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418666.jpg "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human MBNL2 and MBNL3.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 8 seconds.All lanes: Western blot - Anti-MBNL1 antibody [EPR26983-11] (Anti-MBNL1 antibody [EPR26983-11] ab309348) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 10 µg
Lane 2: 293T transfected with MBNL1(WT) expression vector containing a myc-His-tag® whole cell lysate at 10 µg
Lane 3: 293T transfected with MBNL2(WT) expression vector containing a myc-His-tag® whole cell lysate at 10 µg
Lane 4: 293T transfected with MBNL3(WT) expression vector containing a myc-His-tag® whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 42 kDa, 38 kDa, 34 kDa, 26 kDa
Exposure time: 8s
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418665.jpg "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 23949219?PMID:16864772).
Exposure time: Lane 1: 180 seconds; Lane 2: 136 seconds.All lanes: Western blot - Anti-MBNL1 antibody [EPR26983-11] (Anti-MBNL1 antibody [EPR26983-11] ab309348) at 1/1000 dilution
Lane 1: Mouse heart tissue lysate at 20 µg
Lane 2: C2C12 (mouse myoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 42 kDa, 38 kDa, 34 kDa, 26 kDa
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418664.jpg "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)
This data was developed using Anti-MBNL1 antibody [EPR26983-11] ab309348, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lanes 1-3: 6 seconds; Lane 4: 37 seconds.All lanes: Western blot - Anti-MBNL1 antibody [EPR26983-11] (Anti-MBNL1 antibody [EPR26983-11] ab309348) at 1/1000 dilution
Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: Jurkat transfected with siRNA 1 specifically targeti MBNL1 whole cell lysate at 20 µg
Lane 3: Jurkat transfected with siRNA 2 specifically targeti MBNL1 whole cell lysate at 20 µg
Lane 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 42 kDa
![Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--immunoprecipitation-img418674.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418666.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418665.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
![Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349), expandable thumbnail](https://content.abcam.com/products/images/mbnl1-antibody-epr26983-11-bsa-and-azide-free-ab309349--western-blot-img418664.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MBNL1 antibody [EPR26983-11] - BSA and Azide free (ab309349)")
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