AB197590

Anti-MBNL3 antibody [EPR16337]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal MBNL3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CHCR, MBLX39, MBXL, MBNL3, Muscleblind-like protein 3, Cys3His CCG1-required protein, Muscleblind-like X-linked protein, Protein HCHCR

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MBNL3 antibody [EPR16337] (AB197590)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MBNL3 antibody [EPR16337] (AB197590)

ab197590 staining MBNL3 in JAR (human choriocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control : PBS only

Supplier Data

Western blot - Anti-MBNL3 antibody [EPR16337] (AB197590)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression is consistent with what has been described in the literature (PMID : )11929853.

All lanes:

Western blot - Anti-MBNL3 antibody [EPR16337] (ab197590) at 1/1000 dilution

All lanes:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Carrier free

Anti-MBNL3 antibody [EPR16337] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16337

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/800", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscleblind-like protein 3 (MBNL3) mechanically plays a role in RNA splicing and localization. It lacks a popular alternate name in scientific literature. MBNL3 has a mass of 36 kDa and is expressed widely in human tissues with higher expression in skeletal muscle heart and brain. The protein binds RNA via its zinc-finger domains enabling it to influence the processing of pre-mRNA transcripts.

Biological function summary

In the regulation of gene expression networks MBNL3 modulates alternative splicing and RNA stability. Though not part of a larger multiprotein complex it interacts directly with precursor mRNA specifically affecting splicing events. This regulation is critical in maintaining normal tissue function and development particularly in muscle and neural tissues.

Pathways

MBNL3 impacts RNA processing and stress response pathways. MBNL3 interacts with its homologs MBNL1 and MBNL2 which are part of the same splicing regulatory network. Through these interactions it affects pathways involved in cell differentiation and response to environmental stress contributing to the regulation of gene expression under varying physiological conditions.

Defects in MBNL3 function are associated with myotonic dystrophy and certain cancers. In myotonic dystrophy imbalanced splicing caused by malfunction of MBNL proteins including MBNL3 leads to muscular and neurological symptoms. In cancers changes in the expression or activity of MBNL3 might influence tumor progression through its role in splicing regulation. Understanding the connection between MBNL3 and diseases can inform therapeutic target research and potential treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

See full target information MBNL3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40789740

2025

Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatong Xiao,Jinhui Liu,Chunyu Zhang,Zhi Liu,Zhenyu Nie,Zhenglin Yi,Xin Gao,Haisu Liang,Jinliang Huang,Zhiyong Cai,Luzhe Yan,Bingquan Wu,Zefu Liu,Jinbo Chen,Xiongbing Zu,Jiao Hu

Journal of microbiology and biotechnology 34:1433-1442 PubMed38955795

2024

MBNL3 Acts as a Target of miR-302e to Facilitate Cell Proliferation, Invasion and Angiogenesis of Gastric Adenocarcinoma via AKT/VEGFA Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weiping Tang,Can Huang,Bing Jiang,Junjun Lin,Yecai Lu

Cell cycle (Georgetown, Tex.) 19:300-316 PubMed31928130

2020

LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanwu Yu,Gebang Wang,Chenlei Zhang,Yu Liu,Wei Chen,Haoyou Wang,Hongxu Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MBNL3 antibody [EPR16337]

Immunocytochemistry/ Immunofluorescence - Anti-MBNL3 antibody [EPR16337] (AB197590)

Western blot - Anti-MBNL3 antibody [EPR16337] (AB197590)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise