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AB251241

Anti-MBNL3 antibody [EPR16337] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MBNL3 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CHCR, MBLX39, MBXL, MBNL3, Muscleblind-like protein 3, Cys3His CCG1-required protein, Muscleblind-like X-linked protein, Protein HCHCR

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (AB251241)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (AB251241)

This data was developed using ab197590, the same antibody clone in a different buffer formulation.

ab197590 staining MBNL3 in JAR (human choriocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control : PBS only

Western blot - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (AB251241)
  • WB

Supplier Data

Western blot - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (AB251241)

This data was developed using ab197590, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression is consistent with what has been described in the literature (PMID : 11929853).

All lanes:

Western blot - Anti-MBNL3 antibody [EPR16337] (<a href='/en-us/products/primary-antibodies/mbnl3-antibody-epr16337-ab197590'>ab197590</a>) at 1/1000 dilution

All lanes:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16337

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251241 is the carrier-free version of ab197590.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscleblind-like protein 3 (MBNL3) mechanically plays a role in RNA splicing and localization. It lacks a popular alternate name in scientific literature. MBNL3 has a mass of 36 kDa and is expressed widely in human tissues with higher expression in skeletal muscle heart and brain. The protein binds RNA via its zinc-finger domains enabling it to influence the processing of pre-mRNA transcripts.
Biological function summary

In the regulation of gene expression networks MBNL3 modulates alternative splicing and RNA stability. Though not part of a larger multiprotein complex it interacts directly with precursor mRNA specifically affecting splicing events. This regulation is critical in maintaining normal tissue function and development particularly in muscle and neural tissues.

Pathways

MBNL3 impacts RNA processing and stress response pathways. MBNL3 interacts with its homologs MBNL1 and MBNL2 which are part of the same splicing regulatory network. Through these interactions it affects pathways involved in cell differentiation and response to environmental stress contributing to the regulation of gene expression under varying physiological conditions.

Defects in MBNL3 function are associated with myotonic dystrophy and certain cancers. In myotonic dystrophy imbalanced splicing caused by malfunction of MBNL proteins including MBNL3 leads to muscular and neurological symptoms. In cancers changes in the expression or activity of MBNL3 might influence tumor progression through its role in splicing regulation. Understanding the connection between MBNL3 and diseases can inform therapeutic target research and potential treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.
See full target information MBNL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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