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AB140889

Anti-MBOAT4 antibody

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(2 Publications)

Rabbit Polyclonal MBOAT4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MBOAT4.

View Alternative Names

GOAT, OACT4, FKSG89, MBOAT4, Membrane-bound ghrelin O-acyltransferase MBOAT4, Membrane-bound O-acyltransferase domain-containing protein 4, O-acyltransferase domain-containing protein 4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBOAT4 antibody (AB140889)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBOAT4 antibody (AB140889)

Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded Human thyroid medullary carcinoma tissue labelling MBOAT4 with ab140889 at 10 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBOAT4 antibody (AB140889)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBOAT4 antibody (AB140889)

Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded Human stomach tissue labelling MBOAT4 with ab140889 at 10 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MBOAT4. The exact immunogen used to generate this antibody is proprietary information.

Q96T53

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MBOAT4 also known as Membrane-bound O-acyltransferase 4 and Ghrelin O-acyltransferase (GOAT) is an enzyme that catalyzes the octanoylation of ghrelin a process important for its biological activity. MBOAT4 has a mass of approximately 49 kDa and is predominantly expressed in stomach tissues. This enzyme localizes to the endoplasmic reticulum membrane where it functions. Understanding the expression and location of MBOAT4 can provide insights into its roles and mechanisms within the body.
Biological function summary

MBOAT4 plays an important role in energy homeostasis and appetite regulation by modifying ghrelin the hunger hormone. Ghrelin needs acylation for activation and MBOAT4 facilitates this important modification. While this enzyme acts independently it interacts indirectly through its substrate ghrelin within complex metabolic and physiological processes. Because of this role MBOAT4 contributes to the regulation of glucose metabolism and possibly influences lipid metabolism.

Pathways

MBOAT4 participates in the energy balance and glucose metabolism pathways. It acts alongside proteins such as GHSR the ghrelin receptor to influence these pathways. MBOAT4’s presence in these pathways highlights its influence on energy intake and hunger signaling giving it a significant role in maintaining the body's energy balance. It interacts indirectly with insulin pathways due to its effect on ghrelin and energy homeostasis impacting glucose regulation.

MBOAT4 links to obesity and type 2 diabetes due to its function in modifying ghrelin and regulation of appetite. Dysregulation of this enzyme’s activity could lead to altered energy balance and glucose intolerance. MBOAT4 has connections with leptin another hormone involved in appetite control through these metabolic disorders. Understanding MBOAT4's function and its relation to these conditions provides potential targets for therapeutic strategies in managing metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes ghrelin acylation at 'Ser-3' using preferentially octanoyl-CoA, hexanoyl-CoA and decanoyl-CoA as acyl-CoA donors leading to ghrelin activity (PubMed : 18443287, PubMed : 24045953, PubMed : 25562443, PubMed : 28134508). In vitro also uses acyl-CoA donors of different lengths from short-chain (C2) to long-chain fatty acids (C16) knowing that acyl-CoA donors from butanoyl-CoA (C4) to dodecanoyl-CoA (C12) are more efficient compared to longer acyl-CoA donors, such as myristoyl-CoA (C14) and palmitoyl-CoA (C16) that are not efficient (PubMed : 18443287).
See full target information MBOAT4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Comparative biochemistry and physiology. Part A, M 235:112-120 PubMed31158494

2019

Metabolic stress leads to divergent changes in the ghrelinergic system in goldfish (Carassius auratus) gonads.

Applications

Unspecified application

Species

Unspecified reactive species

Jithine Jayakumar Rajeswari,Azadeh Hatef,Mahdi Golshan,Sayyed Mohammad Hadi Alavi,Suraj Unniappan

Scientific reports 7:45024 PubMed28338019

2017

Ghrelin Facilitates GLUT2-, SGLT1- and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus).

Applications

Unspecified application

Species

Unspecified reactive species

Ayelén Melisa Blanco,Juan Ignacio Bertucci,Naresh Ramesh,María Jesús Delgado,Ana Isabel Valenciano,Suraj Unniappan
View all publications
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Product promise

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