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AB248599

Anti-MC-2 antibody [EPR8386] - BSA and Azide free

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Rabbit Recombinant Monoclonal MC-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Melanocortin receptor 5, MC5-R, MC-2, MC5R

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)

This data was developed using ab133656, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling MC-2 with ab133656 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)
  • WB

Unknown

Western blot - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)

This data was developed using ab133656, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MC-2 antibody [EPR8386] (<a href='/en-us/products/primary-antibodies/mc-2-antibody-epr8386-ab133656'>ab133656</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

OI-RD Scanning - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MC-2 antibody [EPR8386] - BSA and Azide free (AB248599)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8386

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab248599 is the carrier-free version of ab133656.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MC2 also known as adrenocorticotropic hormone receptor (ACTHR) plays a critical role in the adrenal gland's function by binding ACTH. This receptor is a G protein-coupled receptor with a mass of approximately 33 kDa. MC2 expression is localized mainly in the adrenal cortex where it facilitates the regulation of adrenal hormone production essential for physiological stress response.
Biological function summary

The adrenocorticotropic hormone receptor mediates the effects of ACTH by stimulating adrenal steroidogenesis. It serves as part of the MC2 receptor complex which includes the MC2 receptor accessory protein (MRAP). This complex is key in MC2's activation and proper targeting to the cell surface important for cortisol production in response to stress stimuli.

Pathways

MC2 is significantly involved in the hypothalamic-pituitary-adrenal (HPA) axis. This pathway regulates stress-induced hormone release where MC2 interacts with proteins such as MRAP to mediate cortisol secretion. The action of MC2 influences the downstream signaling pathways including the cAMP/PKA pathway which plays a pivotal role in hormone synthesis and secretion regulation.

Alterations in MC2 function are linked to conditions such as familial glucocorticoid deficiency type 1 (FGD1) and Cushing's syndrome. Familial glucocorticoid deficiency arises due to MC2 mutations affecting adrenal response leading to insufficient cortisol levels. The receptor's function interplays with MRAP essential for disease manifestation and a potential therapeutic target. Meanwhile Cushing's syndrome characterized by excessive cortisol may involve MC2 dysregulation in adrenal hyperplasia-related mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor for melanocyte-stimulating hormones (alpha- beta- and gamma-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor (PubMed : 37524700, PubMed : 8396929). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway (PubMed : 37524700). Also activates ERK1/2 via a PI3K-dependent signaling mechanism (PubMed : 19428994). Order of potency of natural melanocortins in receptor activation is alpha-MSH > ACTH > beta-MSH > gamma-MSH (PubMed : 8396929). Plays a key role in immune response, and is essential for temperature regulation and exocrine gland function (By similarity).
See full target information MC5R
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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