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AB180776

Anti-MC1-R antibody

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(7 Publications)

Rabbit Polyclonal MC1-R antibody. Suitable for WB and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MC1R.

View Alternative Names

MSHR, MC1R, Melanocyte-stimulating hormone receptor, MSH-R, Melanocortin receptor 1, MC1-R

2 Images
Western blot - Anti-MC1-R antibody (AB180776)
  • WB

Supplier Data

Western blot - Anti-MC1-R antibody (AB180776)

All lanes:

Western blot - Anti-MC1-R antibody (ab180776) at 1/500 dilution

All lanes:

Mouse intestinum tenue tissue lysate

Predicted band size: 35 kDa

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Western blot - Anti-MC1-R antibody (AB180776)
  • WB

CiteAb

Western blot - Anti-MC1-R antibody (AB180776)

MC1-R western blot using anti-MC1-R antibody ab180776. Publication image and figure legend from Wu, X., Fu, S., et al., 2019, J Neuroinflammation, PubMed 31660977.

ab180776 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180776 please see the product overview.

Expression of Mc1r after intracerebral hemorrhage (ICH). a Representative Western blot band and quantitative analyses of Mc1r time-dependent expression from the peri-hematoma tissue after ICH. #p < 0.05 vs sham. n = 6 per group. b Representative brain sample with schematic illustration presenting the four regions in peri-hematoma area (indicated by black boxes). c Representative images of double immunofluorescence staining showed that Mc1r was colocalized with microglia (Iba-1), endothelium (vWF), astrocyte (GFAP), and neuron (NeuN) and at 24 h after ICH. n = 3 per group. Scale bar = 50 μm

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MC1R. The exact immunogen used to generate this antibody is proprietary information.

Q01726

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MC1-R also known as the melanocortin 1 receptor is a protein that plays an important role in the regulation of skin and hair pigmentation. It is a G-protein-coupled receptor with a molecular mass of approximately 37 kDa. The receptor is expressed mainly in melanocytes the cells responsible for producing melanin in the skin and hair. Additionally it appears in other cells such as keratinocytes and certain types of immune cells. By binding specific ligands the MC1-R triggers a cascade of intracellular signals that influence melanin production.
Biological function summary

The MC1-R affects pigmentation by increasing the synthesis of eumelanin the dark pigment in the skin and hair while reducing pheomelanin the red or yellow pigment. It functions as part of a complex with other melanocortin receptors working in coordination to modulate the overall pigmentation process in response to environmental cues like ultraviolet radiation. The receptor achieves this by impacting key enzymes involved in melanin production such as tyrosinase.

Pathways

The MC1-R is part of the melanogenesis pathway a critical process for pigmentation protection against UV radiation and development. It interacts with other melanocortin receptors and is activated by melanocyte-stimulating hormones (MSH). The receptor plays a role in the cyclic adenosine monophosphate (cAMP) signaling pathway where it regulates the activity of the protein kinase A (PKA) an essential step in upregulating melanin synthesis. The cAMP pathway also links MC1-R with other related proteins such as CREB which further regulates gene expression.

MC1-R mutations or dysregulations associate with conditions like melanoma and oculocutaneous albinism. In melanoma alterations in MC1-R signaling can lead to increased susceptibility to UV-induced damage as the protective eumelanin is reduced. The receptor also interacts with the protein p53 well known for its role in tumor suppression highlighting its significance in cancer biology. In oculocutaneous albinism MC1-R dysfunction results in decreased melanin production leading to pigmentation deficits and increased risks of skin cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for MSH (alpha, beta and gamma) and ACTH (PubMed : 11442765, PubMed : 11707265, PubMed : 1325670, PubMed : 1516719, PubMed : 8463333). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed : 11707265, PubMed : 1325670, PubMed : 16463023, PubMed : 19737927). Mediates melanogenesis, the production of eumelanin (black/brown) and phaeomelanin (red/yellow), via regulation of cAMP signaling in melanocytes (PubMed : 31097585).
See full target information MC1R
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:34 PubMed38448811

2024

Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1.

Applications

Unspecified application

Species

Unspecified reactive species

Hengru Guo,Ying Xin,Saifei Wang,Xiaoning Zhang,Yanqi Ren,Bo Qiao,Hongjiang Li,Jing Wu,Xiao Hao,Lijun Xu,Yushan Yan,Haohao Zhang

Animal genetics 53:317-326 PubMed35292981

2022

Functional characterization of cAMP signaling of variant porcine MC1R alleles in PK15 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Lan,Min Wang,Ke Qin,Xiaofeng Liu,Xuan Shi,Guanjie Sun,Xiaohong Liu,Yaosheng Chen,Zuyong He

The American journal of pathology 192:270-280 PubMed34774519

2021

The Neuropeptide Alpha-Melanocyte-Stimulating Hormone Is Critical for Corneal Endothelial Cell Protection and Graft Survival after Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Zala Lužnik Marzidovšek,Tomas Blanco,Zhongmou Sun,Hamid Alemi,Gustavo Ortiz,Hayate Nakagawa,Sunil K Chauhan,Andrew W Taylor,Ula V Jurkunas,Jia Yin,Reza Dana

Theranostics 11:2670-2690 PubMed33456566

2021

Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Fan,Li Zeng,Jing Guo,Shuqin Xie,Yuan Yu,Jianwei Chen,Jin Cao,Qinyanqiu Xiang,Siliang Zhang,Yuanli Luo,Qingyue Deng,Qin Zhou,Yan Zhao,Lan Hao,Zhigang Wang,Ling Zhong

The journal of poultry science 56:84-90 PubMed32055201

2020

Construction of MC1R and ASIP Eukaryotic Expression Vector and its Regulation of Plumage Color in Japanese Quail ().

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Xin Li,Xiao-Hui Zhang,You-Zhi Pang,Yan-Xia Qi,Shu-Juan Zhao

Journal of neuroinflammation 16:192 PubMed31660977

2019

NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-κB pathway after intracerebral hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wu,Siming Fu,Yun Liu,Hansheng Luo,Feng Li,Yiying Wang,Meng Gao,Yuan Cheng,Zongyi Xie

Cell cycle (Georgetown, Tex.) 18:413-424 PubMed30739530

2019

ARPE-19-derived VEGF-containing exosomes promote neovascularization in HUVEC: the role of the melanocortin receptor 5.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Maisto,María Oltra,Lorena Vidal-Gil,Natalia Martínez-Gil,Javier Sancho-Pellúz,Clara Di Filippo,Settimio Rossi,Michele D Amico,Jorge Miguel Barcia,Francisco Javier Romero
View all publications
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