AB125031

Anti-MC1-R antibody [EPR6530]

Name: Anti-MC1-R antibody [EPR6530] (ab125031) | Abcam
Brand: Abcam Limited
SKU: AB125031
Price: 215 USD
Availability: InStock
Rating: 4 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal MC1-R antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

MSHR, MC1R, Melanocyte-stimulating hormone receptor, MSH-R, Melanocortin receptor 1, MC1-R

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (AB125031)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (AB125031)

ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MC1-R antibody [EPR6530] (AB125031)

All lanes:

Western blot - Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

Human melanoma lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 35 kDa

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OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (AB125031)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (AB125031)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MC1-R antibody [EPR6530] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6530

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MC1-R also known as the melanocortin 1 receptor is a protein that plays an important role in the regulation of skin and hair pigmentation. It is a G-protein-coupled receptor with a molecular mass of approximately 37 kDa. The receptor is expressed mainly in melanocytes the cells responsible for producing melanin in the skin and hair. Additionally it appears in other cells such as keratinocytes and certain types of immune cells. By binding specific ligands the MC1-R triggers a cascade of intracellular signals that influence melanin production.

Biological function summary

The MC1-R affects pigmentation by increasing the synthesis of eumelanin the dark pigment in the skin and hair while reducing pheomelanin the red or yellow pigment. It functions as part of a complex with other melanocortin receptors working in coordination to modulate the overall pigmentation process in response to environmental cues like ultraviolet radiation. The receptor achieves this by impacting key enzymes involved in melanin production such as tyrosinase.

Pathways

The MC1-R is part of the melanogenesis pathway a critical process for pigmentation protection against UV radiation and development. It interacts with other melanocortin receptors and is activated by melanocyte-stimulating hormones (MSH). The receptor plays a role in the cyclic adenosine monophosphate (cAMP) signaling pathway where it regulates the activity of the protein kinase A (PKA) an essential step in upregulating melanin synthesis. The cAMP pathway also links MC1-R with other related proteins such as CREB which further regulates gene expression.

MC1-R mutations or dysregulations associate with conditions like melanoma and oculocutaneous albinism. In melanoma alterations in MC1-R signaling can lead to increased susceptibility to UV-induced damage as the protective eumelanin is reduced. The receptor also interacts with the protein p53 well known for its role in tumor suppression highlighting its significance in cancer biology. In oculocutaneous albinism MC1-R dysfunction results in decreased melanin production leading to pigmentation deficits and increased risks of skin cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein (PubMed : 11442765, PubMed : 11707265, PubMed : 1325670, PubMed : 1516719, PubMed : 8463333). Upon activation, MC1R couples with the G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway. This activation promotes melanogenesis, resulting in the production of eumelanin (black/brown) and pheomelanin (red/yellow) in melanocytes (PubMed : 11707265, PubMed : 1325670, PubMed : 16463023, PubMed : 19737927, PubMed : 31097585, PubMed : 34453129). MC1R interacts with G protein-coupled receptor opsin 3/OPN3, which couples to G(i) proteins and inhibits the alpha-MSH-induced cAMP response, thereby reducing melanin synthesis (PubMed : 31097585). Binding to Agouti/ASP precludes alpha-MSH-induced signaling, thereby downregulating melanogenesis (By similarity). Additionally, interaction with MGRN1 displaces the G(s) protein, further suppressing MC1R signaling (PubMed : 19737927).

See full target information MC1R

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Marine drugs 22: PubMed38786597

2024

Anti-Melanogenic Effects of Muscle Hydrolysate in B16F10 Melanoma Cells and Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjin Hu,Bei Chen,Shuaijie Qu,Shuji Liu,Xiaoyu Yang,Kun Qiao,Yongchang Su,Zhihui Liu,Xiaoe Chen,Zhiyu Liu,Qin Wang

JCO precision oncology 8:e2300702 PubMed38662983

2024

Melanocortin-1 Receptor Expression as a Marker of Progression in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

David G Su,Dijana Djureinovic,David Schoenfeld,Bernadette Marquez-Nostra,Kelly Olino,Lucia Jilaveanu,Harriet Kluger

Cancer research 83:1062-1073 PubMed36701140

2023

AMPK Phosphorylates ZDHHC13 to Increase MC1R Activity and Suppress Melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Xin Li,Chengqian Yin,Judy Zhang,Ershang Liang,Xianfang Wu,Ying Ni,Joshua Arbesman,Colin R Goding,Shuyang Chen

Postepy dermatologii i alergologii 38:461-472 PubMed34377129

2021

Modulation of dermal equivalent of hypothalamus-pituitary-adrenal axis in mastocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jakub Antoniewicz,Bogusław Nedoszytko,Magdalena Lange,Justyna Wierzbicka,Magdalena Górska-Ponikowska,Marek Niedoszytko,Monika Zabłotna,Roman J Nowicki,Michał A Żmijewski

Annals of dermatology 32:289-297 PubMed33911756

2020

NB-UVB Induces Melanocytic Differentiation of Human Hair Follicle Neural Crest Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Dong,Shujun Chen,Cheng Feng,Huizi Xiong,Xiaowei Xu

Molecular pharmaceutics 16:3904-3915 PubMed31318566

2019

Enhancing the Efficacy of Melanocortin 1 Receptor-Targeted Radiotherapy by Pharmacologically Upregulating the Receptor in Metastatic Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengshi Li,Dijie Liu,Dongyoul Lee,Somya Kapoor,Katherine N Gibson-Corley,Thomas P Quinn,Edwin A Sagastume,Sarah L Mott,Susan A Walsh,Michael R Acevedo,Frances L Johnson,Michael K Schultz

Nature communications 10:877 PubMed30787281

2019

Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyang Chen,Changpeng Han,Xiao Miao,Xin Li,Chengqian Yin,Junrong Zou,Min Liu,Shanshan Li,Lukasz Stawski,Bo Zhu,Qiong Shi,Zhi-Xiang Xu,Chunying Li,Colin R Goding,Jun Zhou,Rutao Cui

European journal of pharmacology 844:1-8 PubMed30496742

2018

α-melanocyte stimulating hormone (α-MSH) promotes osteoblast differentiation of MC3T3-E1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Jian Cheng,Zhen Hua,Mingming Liu,Haiyan Xu,Yong Ma,Guicheng Huang,Guoqing Mao

ACS applied materials & interfaces 10:4379-4393 PubMed29058865

2018

Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Chen,Xiuli Zhang,Kai Ma,Brian Madajewski,Miriam Benezra,Li Zhang,Evan Phillips,Melik Z Turker,Fabio Gallazzi,Oula Penate-Medina,Michael Overholtzer,Mohan Pauliah,Mithat Gonen,Pat Zanzonico,Ulrich Wiesner,Michelle S Bradbury,Thomas P Quinn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MC1-R antibody [EPR6530]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (AB125031)

Western blot - Anti-MC1-R antibody [EPR6530] (AB125031)

OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (AB125031)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise