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AB313854

Anti-MC1-R antibody [HL1470] - BSA and Azide free

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Rabbit Recombinant Monoclonal MC1-R antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human MC1R conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MSHR, MC1R, Melanocyte-stimulating hormone receptor, MSH-R, Melanocortin receptor 1, MC1-R

4 Images
Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)
  • WB

Supplier Data

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)

All lanes:

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (ab313854) at 1/100000 dilution

Lane 1:

A375 whole cell extract at 30 µg

Lane 2:

MDA-MD-231 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)
  • WB

Supplier Data

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)

Samples were separated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (ab313854) at 1/100000 dilution

Lane 1:

A375 whole cell extracts at 30 µg

Lane 2:

MDA-MD-231 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)
  • WB

Supplier Data

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)

All lanes:

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (ab313854) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract at 50 µg

Lane 2:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)
  • WB

Supplier Data

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (AB313854)

All lanes:

Western blot - Anti-MC1-R antibody [HL1470] - BSA and Azide free (ab313854) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue extract at 50 µg

Lane 2:

Mouse muscle tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1470

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MC1R conjugated to Keyhole Limpet Haemocyanin.

Q01726

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MC1-R also known as the melanocortin 1 receptor is a protein that plays an important role in the regulation of skin and hair pigmentation. It is a G-protein-coupled receptor with a molecular mass of approximately 37 kDa. The receptor is expressed mainly in melanocytes the cells responsible for producing melanin in the skin and hair. Additionally it appears in other cells such as keratinocytes and certain types of immune cells. By binding specific ligands the MC1-R triggers a cascade of intracellular signals that influence melanin production.
Biological function summary

The MC1-R affects pigmentation by increasing the synthesis of eumelanin the dark pigment in the skin and hair while reducing pheomelanin the red or yellow pigment. It functions as part of a complex with other melanocortin receptors working in coordination to modulate the overall pigmentation process in response to environmental cues like ultraviolet radiation. The receptor achieves this by impacting key enzymes involved in melanin production such as tyrosinase.

Pathways

The MC1-R is part of the melanogenesis pathway a critical process for pigmentation protection against UV radiation and development. It interacts with other melanocortin receptors and is activated by melanocyte-stimulating hormones (MSH). The receptor plays a role in the cyclic adenosine monophosphate (cAMP) signaling pathway where it regulates the activity of the protein kinase A (PKA) an essential step in upregulating melanin synthesis. The cAMP pathway also links MC1-R with other related proteins such as CREB which further regulates gene expression.

MC1-R mutations or dysregulations associate with conditions like melanoma and oculocutaneous albinism. In melanoma alterations in MC1-R signaling can lead to increased susceptibility to UV-induced damage as the protective eumelanin is reduced. The receptor also interacts with the protein p53 well known for its role in tumor suppression highlighting its significance in cancer biology. In oculocutaneous albinism MC1-R dysfunction results in decreased melanin production leading to pigmentation deficits and increased risks of skin cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for MSH (alpha, beta and gamma) and ACTH (PubMed : 11442765, PubMed : 11707265, PubMed : 1325670, PubMed : 1516719, PubMed : 8463333). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed : 11707265, PubMed : 1325670, PubMed : 16463023, PubMed : 19737927). Mediates melanogenesis, the production of eumelanin (black/brown) and phaeomelanin (red/yellow), via regulation of cAMP signaling in melanocytes (PubMed : 31097585).
See full target information MC1R

Additional targets

Mc1r
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Product promise

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