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AB77347

Anti-MC2-R antibody

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(3 Publications)

Goat Polyclonal MC2-R antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MC2R aa 1-50.

View Alternative Names

ACTHR, MC2R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTH-R, Adrenocorticotropin receptor, Melanocortin receptor 2, MC2-R

1 Images
Western blot - Anti-MC2-R antibody (AB77347)
  • WB

Unknown

Western blot - Anti-MC2-R antibody (AB77347)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-MC2-R antibody (ab77347) at 1 µg/mL

All lanes:

Human skin lysate in RIPA buffer at 35 µg

Predicted band size: 34 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MC2R aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q01718

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab77347 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The melanocortin 2 receptor (MC2-R) also known as ACTH receptor is a protein that mechanically functions as a G protein-coupled receptor (GPCR). It is responsible for mediating activity through adenylate cyclase signaling by binding with adrenocorticotropic hormone (ACTH). This receptor has a molecular mass of approximately 33 kDa. MC2-R is mostly expressed in the adrenal cortex where it plays a role in synthesizing and releasing glucocorticoids.
Biological function summary

The action of MC2-R initiates the adrenal response to stress through glucocorticoid production which is important for maintaining homeostasis in the body. MC2-R functions as a standalone entity rather than as part of a larger complex. By binding to ACTH MC2-R triggers downstream signaling that leads to steroidogenesis. The biosynthesis of glucocorticoids helps in managing energy immune function and circadian rhythm.

Pathways

MC2-R is integral in the cAMP signaling pathway and the stress response pathway. In the cAMP signaling activation of MC2-R by ACTH results in increased production of cyclic AMP further promoting enzyme activation essential for glucocorticoid synthesis. Its activity is connected to the broader steroid hormone biosynthesis pathway working closely with proteins like StAR (steroidogenic acute regulatory protein) which facilitates cholesterol transport to the inner mitochondrial membrane.

Disruptions in MC2-R function can lead to conditions like familial glucocorticoid deficiency and Cushing's syndrome. Dysfunctional receptor signaling may lead to inadequate glucocorticoid production or excessive hormone levels. The role of MC2-R in these disorders connects it closely with proteins like MC1-R another melanocortin receptor involved in pigmentation and inflammatory response which also signifies related signaling cascade abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor for corticotropin/ACTH, primarily expressed in adrenal cortex where it plays a key role in the regulation of adrenocortical function (PubMed : 1325670, PubMed : 17596328, PubMed : 36588120). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway, the MAPK cascade as well as the PKA pathway, leading to steroidogenic factor 1/NR5A1-mediated transcriptional activation (PubMed : 1325670). Activation by ACTH facilitates the release of adrenal glucocorticoids, including cortisol and corticosterone (PubMed : 12213892, PubMed : 8636348). In addition, MC2R is required for fetal and neonatal adrenal gland development (By similarity).
See full target information MC2R
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 437:312-322 PubMed27524410

2016

Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna M Wierzbicka,Michał A Żmijewski,Anna Piotrowska,Boguslaw Nedoszytko,Magdalena Lange,Robert C Tuckey,Andrzej T Slominski

Journal of cellular physiology 232:154-66 PubMed27061711

2016

Differentiation of Keratinocytes Modulates Skin HPA Analog.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna M Wierzbicka,Michał A Żmijewski,Jakub Antoniewicz,Michal Sobjanek,Andrzej T Slominski

The Journal of clinical investigation 124:1393-405 PubMed24531552

2014

Bitter and sweet taste receptors regulate human upper respiratory innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Lee,Jennifer M Kofonow,Philip L Rosen,Adam P Siebert,Bei Chen,Laurel Doghramji,Guoxiang Xiong,Nithin D Adappa,James N Palmer,David W Kennedy,James L Kreindler,Robert F Margolskee,Noam A Cohen
View all publications
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Product promise

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