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AB230451

Anti-MC3-R antibody

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(1 Publication)

Rabbit Polyclonal MC3-R antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MC3R aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Melanocortin receptor 3, MC3-R, MC3R

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC3-R antibody (AB230451)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC3-R antibody (AB230451)

Formalin-fixed, paraffin-embedded human brain (cortex) tissue stained for MC3 Receptor / MC3-R using ab230451 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC3-R antibody (AB230451)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC3-R antibody (AB230451)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for MC3 Receptor / MC3-R using ab230451 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-MC3-R antibody (AB230451)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MC3-R antibody (AB230451)

Intracellular flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling MC3 Receptor / MC3-R with ab230451 at 1/10 dilution (right histogram) compared to a negetive control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot - Anti-MC3-R antibody (AB230451)
  • WB

Supplier Data

Western blot - Anti-MC3-R antibody (AB230451)

All lanes:

Western blot - Anti-MC3-R antibody (ab230451) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 35 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MC3R aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P41968

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MC3-R also known as melanocortin 3 receptor is a member of the melanocortin receptor family. It functions as a G-protein-coupled receptor with a mass of approximately 35 kDa. MC3-R is expressed mainly in the hypothalamus and pituitary gland with lower expression seen in other regions of the brain and peripheral tissues. It binds to several peptide hormones including alpha- beta- and gamma-melanocyte-stimulating hormones (MSH) and adrenocorticotropic hormone (ACTH).
Biological function summary

MC3-R serves roles in controlling energy homeostasis and inflammatory responses. This receptor is not part of a complex but it contributes to several physiological processes such as modulation of fat storage and glucose metabolism. It influences feeding behavior and energy expenditure by acting on specific neuropeptides. Its activity links to several neuroendocrine functions as well.

Pathways

MC3-R integrates into the central melanocortin system which involves the regulation of energy balance. It operates alongside other melanocortin receptors such as MC4-R to mediate appetite and body weight management. The receptor also participates in the regulation of the hypothalamic-pituitary-adrenal (HPA) axis interacting with proteins like pro-opiomelanocortin (POMC) and Agouti-related peptide (AgRP) in its pathways.

MC3-R connects to obesity and metabolic syndrome. Disruptions in its signaling can lead to abnormal energy balance and weight gain. This receptor also relates to inflammatory diseases as dysregulation of its pathways may contribute to chronic inflammatory conditions. MC4-R and POMC are among the proteins that associate with MC3-R in these disorders reflecting its role in complex physiological and pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor for melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor (PubMed : 37524700, PubMed : 8463333). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which contributes to the regulation of energy homeostasis (PubMed : 18231126, PubMed : 37524700, PubMed : 8463333). Required for expression of anticipatory patterns of activity and wakefulness during periods of limited nutrient availability and for the normal regulation of circadian clock activity in the brain (By similarity). Binding of the Agouti-related protein/AGPR antagonist precludes alpha-MSH-induced signaling, blocking cAMP production (PubMed : 9311920).
See full target information MC3R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 14: PubMed38255741

2024

Spatial Transcriptomic Profiling of Tetraspanins in Stage 4 Colon Cancer from Primary Tumor and Liver Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Thanawat Suwatthanarak,Kullanist Thanormjit,Tharathorn Suwatthanarak,Onchira Acharayothin,Asada Methasate,Vitoon Chinswangwatanakul,Pariyada Tanjak
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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