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AB150419

Anti-MC4-R antibody [EPR6534(2)(ABC)]

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(4 Publications)

Rabbit Recombinant Monoclonal MC4-R antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Melanocortin receptor 4, MC4-R, MC4R

1 Images
Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (AB150419)
  • WB

Unknown

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (AB150419)

All lanes:

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

  • Carrier free

    Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6534(2)(ABC)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The melanocortin-4 receptor also known as MC4-R is a G protein-coupled receptor found within the melanocortin receptor family. It has a molecular mass of approximately 38 kDa. MC4-R is expressed in the central nervous system with high levels in the hypothalamus a region that regulates energy balance and homeostasis. The receptor is activated by several peptide ligands including alpha-melanocyte-stimulating hormone (α-MSH) and it interacts with specific signaling cascades through G proteins.
Biological function summary

The actions through MC4-R are important for energy regulation and appetite control. As part of the melanocortin system MC4-R influences feeding behavior and energy expenditure. Its activation by endogenous or synthetic MC4-R activators suppresses appetite and increases energy consumption. The receptor does not work alone; it forms part of a signaling complex involving other receptor proteins such as MC3-R. The activity of MC4-R also connects to the regulation of body weight and influences fat mass.

Pathways

MC4-R plays an important role in the leptin-melanocortin pathway and the central regulation of energy homeostasis. Leptin a hormone produced by adipose tissues acts upstream by enhancing α-MSH production which in turn activates MC4-R. This activation leads to reduced food intake and increased thermogenesis. Moreover MC4-R interacts with another important signaling protein AGRP (Agouti-related peptide) which acts as an antagonist opposing the effects of α-MSH and regulating energy balance in a complex feedback system.

Mutations or dysregulation of MC4-R are linked to obesity and metabolic syndrome. About 5% of severe early-onset obesity cases involve mutations in the MC4-R gene. The receptor influences the risk for obesity-related conditions through its interactions with AGRP and α-MSH. Additionally compromised MC4-R signaling can lead to disorders such as cachexia where it impacts appetite and metabolism related to conditions like cancer or chronic illness. Defective MC4-R activity therefore is important in understanding the intricate balance between appetite energy expenditure and body mass control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).
See full target information MC4R
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Progress in neurobiology 238:102632 PubMed38821345

2024

A molecularly defined orbitofrontal cortical neuron population controls compulsive-like behavior, but not inflexible choice or habit.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie T Yount,Silu Wang,Aylet T Allen,Lauren P Shapiro,Laura M Butkovich,Shannon L Gourley

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22920 PubMed37078546

2023

Glutamatergic melanocortin-4 receptor neurons regulate body weight.

Applications

Unspecified application

Species

Unspecified reactive species

Haodong Liu,Xiaojing Li,Penghui Li,Rihan Hai,Jiacheng Li,Qi Fan,Xing Wang,Yujie Chen,Xiaojuan Cao,Xiaoyu Zhang,Ruifeng Gao,Kun Wang,Chenguang Du

Frontiers in molecular biosciences 9:787885 PubMed35433838

2022

Proteomic Analysis of Serum Lysine Acetylation in Uyghur Patients With T2DM.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yin,Gang Feng,Chun Huang,Weijuan Cai

Bioengineered 13:5598-5612 PubMed34787056

2021

Demethyltransferase FTO alpha-ketoglutarate dependent dioxygenase (FTO) regulates the proliferation, migration, invasion and tumor growth of prostate cancer by modulating the expression of melanocortin 4 receptor (MC4R).

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Lin Cao
View all publications
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Product promise

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