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AB248980

Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free

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Rabbit Recombinant Monoclonal MC4-R antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

Melanocortin receptor 4, MC4-R, MC4R

1 Images
Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free (AB248980)
  • WB

Unknown

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free (AB248980)

This data was developed using ab150419, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (<a href='/en-us/products/primary-antibodies/mc4-r-antibody-epr65342abc-ab150419'>ab150419</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6534(2)(ABC)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-AMPK alpha 1 antibody [Y365]

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Western blot - Anti-AMPK alpha 1 antibody [Y365] (AB32047)

  • 5
  • 6
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Reactivity data

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Product details

ab248980 is the carrier-free version of ab150419.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The melanocortin-4 receptor also known as MC4-R is a G protein-coupled receptor found within the melanocortin receptor family. It has a molecular mass of approximately 38 kDa. MC4-R is expressed in the central nervous system with high levels in the hypothalamus a region that regulates energy balance and homeostasis. The receptor is activated by several peptide ligands including alpha-melanocyte-stimulating hormone (α-MSH) and it interacts with specific signaling cascades through G proteins.
Biological function summary

The actions through MC4-R are important for energy regulation and appetite control. As part of the melanocortin system MC4-R influences feeding behavior and energy expenditure. Its activation by endogenous or synthetic MC4-R activators suppresses appetite and increases energy consumption. The receptor does not work alone; it forms part of a signaling complex involving other receptor proteins such as MC3-R. The activity of MC4-R also connects to the regulation of body weight and influences fat mass.

Pathways

MC4-R plays an important role in the leptin-melanocortin pathway and the central regulation of energy homeostasis. Leptin a hormone produced by adipose tissues acts upstream by enhancing α-MSH production which in turn activates MC4-R. This activation leads to reduced food intake and increased thermogenesis. Moreover MC4-R interacts with another important signaling protein AGRP (Agouti-related peptide) which acts as an antagonist opposing the effects of α-MSH and regulating energy balance in a complex feedback system.

Mutations or dysregulation of MC4-R are linked to obesity and metabolic syndrome. About 5% of severe early-onset obesity cases involve mutations in the MC4-R gene. The receptor influences the risk for obesity-related conditions through its interactions with AGRP and α-MSH. Additionally compromised MC4-R signaling can lead to disorders such as cachexia where it impacts appetite and metabolism related to conditions like cancer or chronic illness. Defective MC4-R activity therefore is important in understanding the intricate balance between appetite energy expenditure and body mass control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha- and beta-MSH) and corticotropin/ACTH, which are peptide products of the POMC precursor (PubMed : 12646665, PubMed : 14764818, PubMed : 25163632, PubMed : 32327598, PubMed : 33858992, PubMed : 8392067). Functions as a central component of the leptin-melanocortin pathway, which is essential for maintaining energy homeostasis (PubMed : 32327598, PubMed : 33858992). Upon activation, couples to G(s) protein, stimulating adenylate cyclase and the cAMP-dependent signaling pathway, which promotes anorexogenic signaling in the hypothalamus and contributes to a negative energy balance (PubMed : 12588803, PubMed : 14764818, PubMed : 25163632, PubMed : 33858992). Regulates food intake : activation by agonists suppresses appetite, whereas the antagonist Agouti-related protein/AGRP precludes agonist-induced signaling, thereby stimulating appetite (PubMed : 9311920). Modulates the firing activity of neurons in paraventricular nucleus (PVN) of the hypothalamus via alpha-MSH and AGRP regulation of inwardly rectifying potassium channel KCNJ13 closure, independently of G(s) signaling (PubMed : 32327598). In the PVN, also interacts with opsin 3/OPN3, which couples to G(i/o) proteins to inhibit MC4R-mediated cAMP signaling, thereby promoting food intake (PubMed : 39951488). In intestinal epithelial cells, contributes to inhibition of hepatic glucose production via nesfatin-1/NUCB2, leading to increased cAMP levels and glucagon-like peptide 1 (GLP-1) secretion (PubMed : 39562740). Interaction with MGRN1 displaces the G(s) protein, further decreasing MC4R signaling activity (PubMed : 19737927). Also activated by gamma-MSH, though with low potency (PubMed : 8392067).
See full target information MC4R
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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