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AB230344

Anti-MCAF2 antibody

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(1 Publication)

Rabbit Polyclonal MCAF2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATF7IP2 aa 300-350.

View Alternative Names

MCAF2, ATF7IP2, Activating transcription factor 7-interacting protein 2, ATF7-interacting protein 2, MBD1-containing chromatin-associated factor 2

3 Images
Flow Cytometry (Intracellular) - Anti-MCAF2 antibody (AB230344)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCAF2 antibody (AB230344)

Intracellular flow cytometric analysis of CEM cells labeling MCAF2 with ab230344 at 1/10 dilution (green) compared with a negative control control (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCAF2 antibody (AB230344)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCAF2 antibody (AB230344)

Formalin-fixed, paraffin-embedded human testis tissue stained for MCAT2 with ab230344 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MCAF2 antibody (AB230344)
  • WB

Supplier Data

Western blot - Anti-MCAF2 antibody (AB230344)

All lanes:

Western blot - Anti-MCAF2 antibody (ab230344) at 1/1000 dilution

All lanes:

CEM cell lysate at 35 µg

Predicted band size: 76 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ATF7IP2 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q5U623

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCAF2 also known as MBD1-containing chromatin-associated factor 2 or MBD1-interacting protein (MCAF2) is a protein with a mass of approximately 47 kDa. It plays a role in chromatin remodeling by interacting with the methyl-CpG binding domain protein 1 (MBD1). MCAF2 exhibits expression in various tissues with significant levels found in human embryonic kidney cells and fibroblasts. It helps regulate gene expression by recruiting chromatin-modifying enzymes facilitating changes in chromatin structure that can either enhance or hinder transcription.
Biological function summary

MCAF2 functions in the context of DNA methylation and chromatin organization. It is a component of the chromatin-associated complex containing MBD1 that aids in linking DNA methylation status to histone modification important for maintaining gene expression patterns. The function of MCAF2 helps in the organization of euchromatin and heterochromatin regions which is foundational for cellular differentiation processes. MCAF2 collaborates with histone deacetylases (HDACs) to target specific gene loci for transcriptional repression.

Pathways

MCAF2 participates in the regulation of key epigenetic pathways particularly DNA methylation and histone deacetylation. Through its interactions with proteins such as MBD1 and HDACs it contributes to the gene silencing pathways that are important for processes like X-chromosome inactivation and genomic imprinting. MCAF2 plays a role in CpG island methylation pathways influencing the network of proteins involved in transcriptional regulation including those collaborating with histone methyltransferases.

MCAF2 has been implicated in cancers particularly through its involvement in regulating genes associated with cell cycle control and apoptosis. Abnormal expression or function of MCAF2 can disrupt these pathways contributing to tumorigenesis. It also links with neurodevelopmental disorders where disruptions in the epigenetic regulation mechanisms interfere with normal brain development. In both cancer and neurodevelopmental disorders MCAF2 functions closely with MBD1 reflecting a significant interaction in pathogenesis tied to its chromatin remodeling activities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (Probable).
See full target information ATF7IP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of oral & facial pain and headache 39:196-203 PubMed40129438

2025

Loss of SCRG1 in chondrocytes inhibits osteoarthritis by promoting autophagy activity in the temporomandibular joint through inhibition of neurokine receptors.

Applications

Unspecified application

Species

Unspecified reactive species

JiaJun Zhang,LePing Yuan,YanYan Zhang,HaoYang Jin,YeKe Zhao,XiaoKe Zeng,YanHui Zou,KeYu Wang,Xin Nie
View all publications
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