Anti-MCAK antibody

• IP

Supplier Data

Immunoprecipitation - Anti-MCAK antibody (AB264149)

MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab70536, ab264148 and ab264149 at 3 μg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 μg/ml.

Lane 1 : ab70536 IP in HeLa whole cell lysate.
Lane 2 : ab264148 IP in HeLa whole cell lysate.
Lane 3 : ab264149 IP in HeLa whole cell lysate.
Lane 4 : Control IgG.

Exposure time : 10 seconds.

Detection : Chemiluminescence

Lane 1:

Immunoprecipitation - Anti-MCAK antibody (<a href='/en-us/products/primary-antibodies/mcak-antibody-ab264148'>ab264148</a>)

Lane 1:

Immunoprecipitation - Anti-MCAK antibody (ab264149)

Lane 1:

Immunoprecipitation - Anti-MCAK antibody (<a href='/en-us/products/primary-antibodies/mcak-antibody-ab70536'>ab70536</a>)

Predicted band size: 81 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-MCAK antibody (AB264149)

MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264149 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264149 at 1 μg/ml.

Lane 1 : ab264149 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

All lanes:

Immunoprecipitation - Anti-MCAK antibody (ab264149)

Predicted band size: 81 kDa

false

• WB

Supplier Data

Western blot - Anti-MCAK antibody (AB264149)

All lanes:

Western blot - Anti-MCAK antibody (ab264149) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 81 kDa

true

Exposure time: 30s