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AB187652

Anti-MCAK antibody [EPR14838]

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(4 Publications)

Rabbit Recombinant Monoclonal MCAK antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK

5 Images
Western blot - Anti-MCAK antibody [EPR14838] (AB187652)
  • WB

Supplier Data

Western blot - Anti-MCAK antibody [EPR14838] (AB187652)

All lanes:

Western blot - Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 3:

Hela cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 81 kDa

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Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (AB187652)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (AB187652)

Intracellular flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (AB187652)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (AB187652)

Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (AB187652)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (AB187652)

Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

Western blot - Anti-MCAK antibody [EPR14838] (AB187652)
  • WB

Lab

Western blot - Anti-MCAK antibody [EPR14838] (AB187652)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : MCAK knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HEK293 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MCAK antibody [EPR14838] (ab187652)

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14838

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/260", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.
Biological function summary

The MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.

Pathways

MCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.

Aberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed : 21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed : 19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed : 23891108).
See full target information KIF2C
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 22:666 PubMed35715760

2022

CKAP2L, a crucial target of miR-326, promotes prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Li,Mo Yan,Chunhui Wang,Kaibin Wang,Guochang Bao

Nature communications 12:1213 PubMed33619254

2021

Chromosomally unstable tumor cells specifically require KIF18A for proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Carolyn Marquis,Cindy L Fonseca,Katelyn A Queen,Lisa Wood,Sarah E Vandal,Heidi L H Malaby,Joseph E Clayton,Jason Stumpff

The Journal of cell biology 219: PubMed32027339

2020

Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Hadders,Sanne Hindriksen,My Anh Truong,Aditya N Mhaskar,J Pepijn Wopken,Martijn J M Vromans,Susanne M A Lens

Scientific reports 5:15464 PubMed26494317

2015

Overshoot during phenotypic switching of cancer cell populations.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro L Sellerio,Emilio Ciusani,Noa Bossel Ben-Moshe,Stefania Coco,Andrea Piccinini,Christopher R Myers,James P Sethna,Costanza Giampietro,Stefano Zapperi,Caterina A M La Porta
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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