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Rabbit Recombinant Monoclonal MCAK antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931), expandable thumbnail
  • Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

5 products for Alternative Version

Target data

Function

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).

Alternative names

KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK

Recommended products

Rabbit Recombinant Monoclonal MCAK antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14838
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250931 is the carrier-free version of Anti-MCAK antibody [EPR14838] ab187652.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.

Biological function summary

The MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.

Pathways

MCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.

Associated diseases and disorders

Aberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931), expandable thumbnail

    Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using Anti-MCAK antibody [EPR14838] ab187652, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)

    Lane 2: MCAK knockout HAP1 whole cell lysate (20 μg)

    Lane 3: HeLa whole cell lysate (20 μg)

    Lane 4: HEK293 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-MCAK antibody [EPR14838] ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Anti-MCAK antibody [EPR14838] ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Anti-MCAK antibody [EPR14838] ab187652 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MCAK antibody [EPR14838] (Anti-MCAK antibody [EPR14838] ab187652)

    Predicted band size: 81 kDa

  • Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using Anti-MCAK antibody [EPR14838] ab187652, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of HeLa cells labeling MCAK with Anti-MCAK antibody [EPR14838] ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

  • Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931), expandable thumbnail

    Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using Anti-MCAK antibody [EPR14838] ab187652, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MCAK antibody [EPR14838] (Anti-MCAK antibody [EPR14838] ab187652) at 1/1000 dilution

    Lane 1: MCF7 cell lysate at 20 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

    Lane 3: Hela cell lysate at 20 µg

    Lane 4: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 81 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using Anti-MCAK antibody [EPR14838] ab187652, the same antibody clone in a different buffer formulation.Immunofluorscence analysis of MCF7 cells labeling MCAK with Anti-MCAK antibody [EPR14838] ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using Anti-MCAK antibody [EPR14838] ab187652, the same antibody clone in a different buffer formulation.Immunofluorscence analysis of HeLa cells labeling MCAK with Anti-MCAK antibody [EPR14838] ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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