AB250931

Anti-MCAK antibody [EPR14838] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal MCAK antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

This data was developed using ab187652, the same antibody clone in a different buffer formulation.

Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

This data was developed using ab187652, the same antibody clone in a different buffer formulation.

Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

Supplier Data

Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

This data was developed using ab187652, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MCAK antibody [EPR14838] (<a href='/en-us/products/primary-antibodies/mcak-antibody-epr14838-ab187652'>ab187652</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 3:

Hela cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 81 kDa

true

Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

This data was developed using ab187652, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

Lab

Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

This data was developed using ab187652, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : MCAK knockout HAP1 whole cell lysate (20 μg)

Lane 3 : HeLa whole cell lysate (20 μg)

Lane 4 : HEK293 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. ab187652 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MCAK antibody [EPR14838] (<a href='/en-us/products/primary-antibodies/mcak-antibody-epr14838-ab187652'>ab187652</a>)

Predicted band size: 81 kDa

false

Unconjugated

Anti-MCAK antibody [EPR14838]
660 APC

APC Anti-MCAK antibody [EPR14838]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MCAK antibody [EPR14838]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MCAK antibody [EPR14838]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-MCAK antibody [EPR14838]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-MCAK antibody [EPR14838]
578 PE

PE Anti-MCAK antibody [EPR14838]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-MCAK antibody [EPR14838]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14838

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250931 is the carrier-free version of ab187652.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.

Biological function summary

The MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.

Pathways

MCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.

Aberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed : 21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed : 19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed : 23891108).

See full target information KIF2C

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCAK antibody [EPR14838] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (AB250931)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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