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AB125920

Anti-MCC antibody

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(1 Publication)

Rabbit Polyclonal MCC antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCC aa 1-350.

View Alternative Names

Colorectal mutant cancer protein, Protein MCC, MCC

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCC antibody (AB125920)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCC antibody (AB125920)

ab125920, at 1/250 dilution, staining MCC in Paraffin-embedded Human Colon carcinoma tissue by immunohistochemistry.

Western blot - Anti-MCC antibody (AB125920)
  • WB

Unknown

Western blot - Anti-MCC antibody (AB125920)

7.5% SDS PAGE

All lanes:

Western blot - Anti-MCC antibody (ab125920) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCC aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P23508

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCC also known as mutated in colorectal cancer is a protein involved in various cellular processes. The MCC protein has a mass of approximately 97 kDa. It expresses in several tissues with notable expression in the colon and liver. MCC is located in the cytoplasm and can translocate to the nucleus. It is linked with cell cycle regulation and likely acts as a tumor suppressor.
Biological function summary

The protein serves multiple roles in cellular homeostasis and growth control. MCC interacts with other proteins to influence cell proliferation apoptosis and differentiation. It forms part of a complex involved in transcriptional regulation binding directly to DNA to control gene expression. Therefore MCC impacts cellular response to environmental signals and maintains genomic stability.

Pathways

MCC engages in the Wnt signaling and cell cycle pathways which are important for its tumor suppressor functions. In the Wnt signaling pathway MCC interacts with β-catenin and can inhibit its activity affecting gene transcription associated with cell growth. In the cell cycle pathway MCC associates with cyclin-dependent kinases (CDKs) regulating their activity to control cell division.

MCC links strongly to colorectal cancer and certain liver pathologies. Mutations or changes in the expression of MCC are implicated in the progression of colorectal cancer where it fails to regulate β-catenin properly. In liver disorders altered MCC expression may relate to disrupted cellular growth control connecting it with proteins like β-catenin and CDKs. These interactions point to its significance in cancer biology and potentially other proliferative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation (PubMed : 18591935, PubMed : 19555689, PubMed : 22480440). Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity (PubMed : 24824780).
See full target information MCC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3481 PubMed34108482

2021

TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Ou,Jia-Qi Fang,Zhe-Sheng Zhang,Zhe Chi,Jie Fang,Di-Yan Xu,Kai-Zhong Lu,Meng-Qing Qian,Da-Yong Zhang,Jun-Ping Guo,Wei Gao,Na-Ru Zhang,Jian-Ping Pan
View all publications
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