AB236397

Anti-MCEE antibody [OTI1B5]

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(2 Publications)

Mouse Monoclonal MCEE antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCEE.

View Alternative Names

DL-methylmalonyl-CoA racemase, MCEE

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human prostate carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human pancreas carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human bladder tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human prostate tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (AB236397)

Paraffin-embedded human colon adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

Western blot - Anti-MCEE antibody [OTI1B5] (AB236397)

WB

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Western blot - Anti-MCEE antibody [OTI1B5] (AB236397)

All lanes:

Western blot - Anti-MCEE antibody [OTI1B5] (ab236397) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY MCEE cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B5

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

Recombinant Full Length Protein corresponding to Human MCEE.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by Tris-EDTA, pH 8.0.</p>" } } }

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Product details

Clone OTI1B5 (formerly 1B5).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCEE also known as methylmalonyl-CoA epimerase is an important enzyme with a mass of approximately 17 kDa. This enzyme is found in the mitochondria and expressed widely across different tissue types. MCEE plays a mechanical role in the conversion of D-methylmalonyl-CoA to L-methylmalonyl-CoA a necessary step in the metabolism of certain fatty acids and amino acids. The proper functioning of this enzyme is essential for efficient metabolic pathways and energy conversion in cells.

Biological function summary

Methylmalonyl-CoA epimerase contributes to the metabolism by facilitating the reversible epimerization process of methylmalonyl-CoA. MCEE is part of an enzymatic complex alongside methylmalonyl-CoA mutase which further processes the epimerized product to succinyl-CoA. This pathway is important for the breakdown and utilization of odd-chain fatty acids and certain branched-chain amino acids feeding into the citric acid cycle which is a critical pathway for cellular energy production.

Pathways

Methylmalonyl-CoA epimerase finds its place in the catabolism of β-oxidation and is directly involved in the propionic acid pathway. This positions MCEE as an integral part in facilitating the conversion of lipids and branched-chain amino acids into energy. It works closely with methylmalonyl-CoA mutase which acts downstream linking MCEE to broader metabolic processes and ensuring the continuation of energy supply within the metabolic network. This relationship is important for maintaining cellular homeostasis particularly in energy-demanding tissues.

Disruptions in the function of methylmalonyl-CoA epimerase can relate to metabolic disorders like methylmalonic acidemia. This condition arises from the accumulation of methylmalonic acid due to ineffective conversion because of enzyme defects. MCEE shows connection with proteins like methylmalonyl-CoA mutase which when dysfunctional exacerbates the metabolic imbalance. Additionally MCEE defects may influence disorders related to oxidative stress as improper metabolite handling affects energy production and cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Methylmalonyl-CoA epimerase involved in propionyl-CoA metabolism.

See full target information MCEE

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Digestive diseases and sciences 70:1395-1410 PubMed39961962

2025

LRRC25 Is a Potential Biomarker for Predicting Immunotherapy Response in Patients with Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhensong Yang,Bin Yu,Jinchen Hu,Lixin Jiang,Mi Jian

Aging 15:3807-3825 PubMed37166429

2023

Identification and validation of metabolism-related genes signature and immune infiltration landscape of rheumatoid arthritis based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Guo,Yuanye Ma,Yaqing Wang,Hongfei Xiang,Huifei Cui,Zuoran Fan,Youfu Zhu,Dongming Xing,Bohua Chen,Hao Tao,Zhu Guo,Xiaolin Wu

View all publications

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Product promise

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