Rabbit Recombinant Monoclonal MCH antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-frozen-sections-img113730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124405.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)")
![Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-frozen-sections-img5457.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38024.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Common marmoset | Not recommended | Not recommended | Not recommended | Predicted | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Common marmoset | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Common marmoset | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Common marmoset | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Rat | Dilution info 1/500 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
Target data
Function
MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
Alternative names
Pro-MCH, MCH, PMCH
Recommended products
Rabbit Recombinant Monoclonal MCH antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23778-94
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognizes the PMCH chain.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Melanin-concentrating hormone (MCH) originally isolated from fish is a peptide hormone that plays a significant role in energy balance and feeding behavior. It is about 19 amino acids long in humans and has a molecular mass of approximately 2.4 kDa. MCH is mainly expressed in the lateral hypothalamus and zona incerta of the brain which suggests its central role in neurologic function. This peptide binds to MCH receptor 1 (MCHR1) influencing various signaling pathways.
- Biological function summary
MCH affects a range of physiological processes and is part of the neuropeptide signaling system. It regulates feeding and energy homeostasis by influencing appetite increase and reducing energy expenditure. MCH operates within a complex network of neurotransmitters and hormones in the central nervous system. Its interactions with other neuropeptides such as neuropeptide Y (NPY) and agouti-related peptide (AgRP) support its role in the complex regulation of energy homeostasis.
- Pathways
MCH engages in signaling linked to G-protein coupled receptors (GPCRs) connecting with pathways that regulate food intake and energy management. It influences the orexin/hypocretin pathway which also modulates sleep-wake cycles and arousal states. The interaction with orexin highlights MCH's broad regulatory influence balancing energy levels and adaptive responses. The protein's signaling is associated with molecules like leptin and insulin marking its integration into wider metabolic pathways.
- Associated diseases and disorders
MCH is linked to obesity and mood disorders due to its influence on appetite and emotional regulation. A dysregulated MCH signaling pathway is associated with overeating and weight gain highlighting its role in obesity physiology. Additionally altered levels of MCH could contribute to mood disorders such as depression where its interaction with serotonin and dopamine pathways may impact emotional stability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-frozen-sections-img113730.jpg "Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)")
Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124405.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-frozen-sections-img5457.jpg "Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)")
Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38024.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415), expandable thumbnail](https://content.abcam.com/products/images/mch-antibody-epr23778-94-ab274415--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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