Anti-mCherry antibody [1C51]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (AB125096)

Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, dilution 1/2000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. ab125096 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (AB125096)

mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.

• IHC-P

AbReview34312****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (AB125096)

ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.

Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor^® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.

Image courtesy of an anonymous Abreview

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody [1C51] (AB125096)

4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.

Analysis of rin hypomorphic alleles and the genomic rescue transgene Grin^Cherry. (A–C'') Negatively marked 72 h old rin² (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the Grin^Cherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 μm.

Baumgartner R et al. The RNA-binding proteins FMR1, rasputin and caprin act together with the UBA protein lingerer to restrict tissue growth in Drosophila melanogaster. PLoS Genet 9:e1003598 (2013).

• WB

Supplier Data

Western blot - Anti-mCherry antibody [1C51] (AB125096)

The tdTomato construct tested is identical to that found in several widely used expression vectors and contains two identical fluorescent modules, explaining why it is twice a large as the mCherry construct which contains only one fluorescent module.

ab125096 antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, in green, respectfully.

The red band at 37kDa corresponds to GAPDH protein used as a loading control.

All lanes:

Western blot - Anti-mCherry antibody [1C51] (ab125096) at 1/2000 dilution

Lane 2:

Non-transfected HEK293 control cells

Lane 3:

HEK293 cells transfected with pCI-Neo-mod vector expressing tdTomato protein

Lane 4:

HEK293 cells transfected with pCI-Neo-mod vector expressing mCherry-HA protein

Observed band size: 60 kDa,30 kDa

false

• WB

Supplier Data

Western blot - Anti-mCherry antibody [1C51] (AB125096)

Major band at about 30kDa corresponds to mCherry protein.

MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1 : 5,000 in red which reveals band at 60kDa seen only in cell lysates.

All lanes:

Western blot - Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution

Lane 1:

Protein standard

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 3:

HEK-293 cells transfected with mCherry-HA construct

Lane 4:

mCherry recombinant protein

Lane 5:

GFP recombinant protein

Lane 6:

HEK-293 transfected with GFP construct.

false