Anti-mCherry antibody is a rabbit polyclonal antibody that is used to detect mCherry in ICC/IF, Western blot.
- Cited in over 470 publications
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- Nature communications 14:84202023Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin ChanPubMed 38110419
- Nature communications 14:83342023Ubiquitination-mediated Golgi-to-endosome sorting determines the toxin-antidote duality of fission yeast wtf meiotic drivers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Xin Zheng,Tong-Yang Du,Guang-Can Shao,Zhu-Hui Ma,Zhao-Di Jiang,Wen Hu,Fang Suo,Wanzhong He,Meng-Qiu Dong,Li-Lin DuPubMed 38097609
- Science advances 9:eadl21082023RAD54L2 counters TOP2-DNA adducts to promote genome stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppina D'Alessandro,David A Morales-Juarez,Sean L Richards,Karin C Nitiss,Almudena Serrano-Benitez,Juanjuan Wang,John C Thomas,Vipul Gupta,Andrea Voigt,Rimma Belotserkovskaya,Chen Gang Goh,Anne Ramsay Bowden,Yaron Galanty,Petra Beli,John L Nitiss,Guido Zagnoli-Vieira,Stephen P JacksonPubMed 38055822
- The Journal of clinical investigation 134:2023AEP-cleaved DDX3X induces alternative RNA splicing events to mediate cancer cell adaptation in harsh microenvironments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenrui Zhang,Lu Cao,Jian Yang,Shuai Zhang,Jianyi Zhao,Zhonggang Shi,Keman Liao,Haiwei Wang,Binghong Chen,Zhongrun Qian,Haoping Xu,Linshi Wu,Hua Liu,Hongxiang Wang,Chunhui Ma,Yongming Qiu,Jianwei Ge,Jiayi Chen,Yingying LinPubMed 37988165
- iScience 26:1084132023Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCollyn M Kellogg,Kevin Pham,Sunghwan Ko,Jillian E J Cox,Adeline H Machalinski,Michael B Stout,Amanda L Sharpe,Michael J Beckstead,Ana J Chucair-Elliott,Sarah R Ocañas,Willard M FreemanPubMed 38058312
- eLife 12:2023Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex W Chan,Malgorzata Broncel,Eden Yifrach,Nicole R Haseley,Sundeep Chakladar,Elena Andree,Alice L Herneisen,Emily Shortt,Moritz Treeck,Sebastian LouridoPubMed 37933960
- Nature neuroscience 27:78-892023Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Pallucchi,Maria Bertuzzi,David Madrid,Pierre Fontanel,Shin-Ichi Higashijima,Abdeljabbar El ManiraPubMed 37919423
- Nature communications 14:67582023A thalamic-hippocampal CA1 signal for contextual fear memory suppression, extinction, and discrimination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeather C Ratigan,Seetha Krishnan,Shai Smith,Mark E J SheffieldPubMed 37875465
- iScience 26:1083062023Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei WangPubMed 38026209
- Nature communications 14:66022023Adrenergic modulation of melanocortin pathway by hunger signals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNilufer Sayar-Atasoy,Connor Laule,Iltan Aklan,Hyojin Kim,Yavuz Yavuz,Tayfun Ates,Ilknur Coban,Fulya Koksalar-Alkan,Jacob Rysted,Debbie Davis,Uday Singh,Muhammed Ikbal Alp,Bayram Yilmaz,Huxing Cui,Deniz AtasoyPubMed 37857606
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.065% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Tag | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500 | Notes - |
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Anti-mCherry antibody is a rabbit polyclonal antibody that is used to detect mCherry in ICC/IF, Western blot.
- Cited in over 470 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.065% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Protein A
- Specificity
Sequence homology shows that this antibody cross-reacts with tdTomato and mOrange
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-mCherry antibody (ab167453) is a rabbit polyclonal antibody and is validated for use in ICC/IF and WB.
Anti-mCherry antibody (ab167453) was first used in a scientific publication in 2014 and has been cited over 471 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-mCherry antibody (ab167453) has high sensitivity and specificity.
Anti-mCherry antibody (ab167453) has 17 independent reviews from customers.
Anti-mCherry antibody (ab167453) specifically detects mCherry (UniProt ID: Q9U6Y8; Molecular weight: 26kDa) and is sold in 100 µL selling sizes.
Top cited antibody on the market to mCherry with >680 citations. mCherry antibody has >17 five star reviews. mCherry antibody is essential for detecting mCherry-tagged proteins in various applications, including western blotting and immunofluorescence ensuring accurate and versatile research
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCherry also known as mCherry fluorescent protein is a widely used red fluorescent protein derived from a coral of the Discosoma species. It has a molecular weight of approximately 29 kDa. Scientists often use mCherry as a tag in various applications. It expresses in various organisms making it highly versatile for visualizing proteins within live cells or whole organisms. The mCherry protein size and its bright red color facilitate the detection and tracking of protein localization and dynamics in complex biological environments. It plays significant roles in cellular imaging and labeling.
- Biological function summary
The mCherry fluorescent protein functions as a reporter providing real-time data on protein localization and gene expression. It is part of a collection of fluorescent proteins used for multi-color labeling. Researchers use mCherry in conjunction with other fluorescent reporters due to its distinct emission spectrum. It does not naturally occur with intrinsic biological activities but rather as an imaging tool. mCherry combines with proteins or structures of interest allowing scientists to examine cellular events with precision.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)
Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
Western blot - Anti-mCherry antibody (ab167453)
All lanes: Western blot - Anti-mCherry antibody (ab167453) at 1/1000 dilution
Lane 1: HEK293 cells transfected with pFin- EF1-mCherry vector
Lane 2: Non-transfected HEK293 cells
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