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Anti-mCherry antibody ab183628 is a rabbit polyclonal antibody that is used in mCherry western blotting and immunofluorescence.

Same trusted quality, new lower price


Images

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB183628), expandable thumbnail
  • IHC - Wholemount - Anti-mCherry antibody (AB183628), expandable thumbnail
  • Western blot - Anti-mCherry antibody (AB183628), expandable thumbnail
  • Immunoprecipitation - Anti-mCherry antibody (AB183628), expandable thumbnail
  • Western blot - Anti-mCherry antibody (AB183628), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC - Wmt
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Tested
Tested
Tested
Tested

Tested
Tested

Species

Tag

Dilution info

1/100 - 1/500

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/100 - 1/1000

Notes

-

Tested
Tested

Species

Tag

Dilution info

1/100 - 1/500

Notes

-

Associated Products

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Anti-mCherry antibody ab183628 is a rabbit polyclonal antibody that is used in mCherry western blotting and immunofluorescence.

Same trusted quality, new lower price

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628), expandable thumbnail
    Wang et al PLoS Pathog. 2018 Jul 20;14(7):e1007189. doi: 10.1371/journal.ppat.1007189. eCollection 2018 Jul. Fig 7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628)

    RABV and mGluR2 are internalized into cells and transported together in early and late endosomes.

    N2a (Mouse neuroblastoma cells) cells were stained by using the Tyramide Signal Amplification immunofluorescent method. N2a cells infected with ERA-N-mCherry for 20 minutes at 37°C were used to perform immunofluorescence staining for RABV antigen (red), mGluR2 (green), Rab5 or Rab7 (purple), and the cell nuclei (blue). Co-localization of the RABV-mGluR2 complex with Rab5 (E, F).

  • IHC - Wholemount - Anti-mCherry antibody (ab183628), expandable thumbnail

    IHC - Wholemount - Anti-mCherry antibody (ab183628)

    Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labeling mCherry-expressing cells with ab183628 at 1/100 dilution.

  • Western blot - Anti-mCherry antibody (ab183628), expandable thumbnail

    Western blot - Anti-mCherry antibody (ab183628)

    All lanes: Western blot - Anti-mCherry antibody (ab183628) at 1/2000 dilution

    Lane 1: Full length recombinant mCherry at 0.005 µg

    Lane 2: Full length recombinant mCherry at 0.01 µg

    Lane 3: Full length recombinant mCherry at 0.02 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 27 kDa

  • Immunoprecipitation - Anti-mCherry antibody (ab183628), expandable thumbnail

    Immunoprecipitation - Anti-mCherry antibody (ab183628)

    Immunoprecipitation analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract using ab183628 at a 1/3000 dilution.

    Lane 1: 20 μg whole cell lysate/extract of Cherry protein-expressing HEK-293T cells.
    Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
    Lane 3: Immunoprecipitation of mCherry protein with 2.5 μg of ab183628.

    7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.

    All lanes: Immunoprecipitation - Anti-mCherry antibody (ab183628)

    Developed using the ECL technique.

  • Western blot - Anti-mCherry antibody (ab183628), expandable thumbnail

    Western blot - Anti-mCherry antibody (ab183628)

    All lanes: Western blot - Anti-mCherry antibody (ab183628) at 1/5000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 30 µg

    Lane 2: Whole cell lysate/extract of mCherry-tagged protein expressing HEK-293T cells at 30 µg

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628)

    Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.

    Red: mCherry fusion protein is expressed in the transfected cell.
    Blue: Hoechst 33342 staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab183628)

    Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.

    Red: mCherry fusion protein is expressed in the transfected cell.
    Blue: Hoechst 33342 staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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