Anti-mCherry chicken polyclonal antibody that is used to detect mCherry in Western blot, ICC/IF. Suitable for samples expresing mCherry tag.
- Cited in over 70 publications
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- Frontiers in molecular neuroscience 16:12949942023Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaphaëlle Quillet,Maria Gutierrez-Mecinas,Erika Polgár,Allen C Dickie,Kieran A Boyle,Masahiko Watanabe,Andrew J ToddPubMed 38143564
- eLife 12:2023Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMastura Akter,Mahadi Hasan,Aruna Surendran Ramkrishnan,Zafar Iqbal,Xianlin Zheng,Zhongqi Fu,Zhuogui Lei,Anwarul Karim,Ying LiPubMed 37960975
- Epigenetics & chromatin 16:452023Differential usage of DNA modifications in neurons, astrocytes, and microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyla B Tooley,Ana J Chucair-Elliott,Sarah R Ocañas,Adeline H Machalinski,Kevin D Pham,Walker Hoolehan,Adam M Kulpa,David R Stanford,Willard M FreemanPubMed 37953264
- Life science alliance 6:2023MAST4 promotes primary ciliary resorption through phosphorylation of Tctex-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKensuke Sakaji,Sara Ebrahimiazar,Yasuhiro Harigae,Kenichi Ishibashi,Takeya Sato,Takeo Yoshikawa,Gen-Ichi Atsumi,Ching-Hwa Sung,Masaki SaitoPubMed 37726137
- The Journal of physiology 601:4309-43362023Paraventricular nucleus projections to the nucleus tractus solitarii are essential for full expression of hypoxia-induced peripheral chemoreflex responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian C Ruyle,Ludmila Lima-Silveira,Diana Martinez,Kevin J Cummings,Cheryl M Heesch,David D Kline,Eileen M HasserPubMed 37632733
- Biomedicines 11:2023Deletion of via Systemic Administration of AAV-PHP.eB Virus Increases Social Behavior in a Mouse Model of a Neurodevelopmental Disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Ophir,Gilad Levy,Ela Bar,Omri Kimchi Feldhorn,May Rokach,Galit Elad Sfadia,Boaz BarakPubMed 37626769
- Nature communications 14:14922023A melanopsin ganglion cell subtype forms a dorsal retinal mosaic projecting to the supraoptic nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael H Berry,Michael Moldavan,Tavita Garrett,Marc Meadows,Olga Cravetchi,Elizabeth White,Joseph Leffler,Henrique von Gersdorff,Kevin M Wright,Charles N Allen,Benjamin SivyerPubMed 36932080
- Nature communications 14:10282023Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanming Ren,Zongyao Huang,Lingling Zhou,Peng Xiao,Junwei Song,Ping He,Chuanxing Xie,Ran Zhou,Menghan Li,Xiangqun Dong,Qing Mao,Chao You,Jianguo Xu,Yanhui Liu,Zhigang Lan,Tiejun Zhang,Qi Gan,Yuan Yang,Tengyun Chen,Bowen Huang,Xiang Yang,Anqi Xiao,Yun Ou,Zhengzheng Su,Lu Chen,Yan Zhang,Yan Ju,Yuekang Zhang,Yuan WangPubMed 36823172
- eLife 12:2023Bilateral JNK activation is a hallmark of interface surveillance and promotes elimination of aberrant cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepti Prasad,Katharina Illek,Friedericke Fischer,Katrin Holstein,Anne-Kathrin ClassenPubMed 36744859
- International journal of molecular sciences 24:2023Different Contacted Cell Types Contribute to Acquiring Different Properties in Brain Microglial Cells upon Intercellular Interaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkiko Nakano-Doi,Shuji Kubo,Emiko Sonoda,Akihiko Taguchi,Takayuki NakagomiPubMed 36675286
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.0325% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Tag | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000 | Notes - |
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Target data
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Anti-mCherry chicken polyclonal antibody that is used to detect mCherry in Western blot, ICC/IF. Suitable for samples expresing mCherry tag.
- Cited in over 70 publications
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
Preservative: 0.0325% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- IgY fraction
- Concentration
- Purification notes
Concentrated IgY preparation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-mCherry antibody (ab205402) is a chicken polyclonal antibody and is validated for use in ICC/IF, WB.
mCherry antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-mCherry antibody (ab205402) has been validated in ICC/IF, WB
Anti-mCherry antibody (ab205402) specifically detects mCherry (UniProt ID: Q9U6Y8; Molecular weight: 26kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-mCherry antibody (ab205402) has high sensitivity and specificity.
Anti-mCherry antibody (ab205402) has been cited over 73 times in peer reviewed journals and is trusted by the scientific community.
Anti-mCherry antibody (ab205402) has 5 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab205402)
Immunofluorescent analysis of HEK293 cells transfected with pFin-EF1-mCherry vector labeling mCherry using ab205402 at 1/1000 dilution (green). Red: mCherry; Blue: blue DNA stain. Note: Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal.
Western blot - Anti-mCherry antibody (ab205402)
All lanes: Western blot - Anti-mCherry antibody (ab205402) at 1/2000 dilution
All lanes: lysate of HEK293 cells transfected with pFin-EF1-mCherry vector
Predicted band size: 30 kDa
Observed band size: 30 kDa
This image is courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab205402)
Immunocytochemistry/ Immunofluorescence analysis of Human ES cells with mCherry gene reporter labeling mCherry with ab205402 at 1/1000 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X. Blocking was carried out with 10%BSA for 1 hour at 4°C, followed by incubation with ab205402 at 1/1000 for 12 hours at 4°C. A polyclonal goat anti-chicken Alexa Fluor® 488 was used as the secondary antibody at 1/100 diluton.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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