Chicken Recombinant Monoclonal mCherry antibody. Carrier free. Suitable for WB and reacts with Tag samples.
Images
![Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (AB316978), expandable thumbnail](https://content.abcam.com/products/images/mcherry-antibody-epr20579-chicken-igy-chimeric-bsa-and-azide-free-ab316978--western-blot-img432968.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (AB316978)")
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | |
|---|---|---|
| Tag | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Target data
Recommended products
Chicken Recombinant Monoclonal mCherry antibody. Carrier free. Suitable for WB and reacts with Tag samples.
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20579
- Purification technique
Affinity purification Thiophilic Resin
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
This chicken monoclonal chimeric antibody (ab316978) is the carrier-free version of Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) ab316977.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (ab316978), expandable thumbnail](https://content.abcam.com/products/images/mcherry-antibody-epr20579-chicken-igy-chimeric-bsa-and-azide-free-ab316978--western-blot-img432968.jpg "Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (ab316978)")
Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) - BSA and Azide free (ab316978)
This data was developed using Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) ab316977, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) ab316977) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag® whole cell lysate at 20 µg
Lane 2: 293T transfected with mCherry expression vector containing a myc-His-tag® whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (Goat Anti-Chicken IgY H&L (HRP) preadsorbed ab7118) at 1/20000 dilution
Observed band size: 27 kDa, 36 kDa, 35 kDa
Exposure time: 10s
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Downloads
Product protocols
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