JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313858

Anti-mCherry antibody [HL1482] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal mCherry antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Tag samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)

Immunofluorescent analysis of mock and mCherry transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling mCherry with ab313858 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)
  • WB

Supplier Data

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (ab313858) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)
  • WB

Supplier Data

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (AB313858)

All lanes:

Western blot - Anti-mCherry antibody [HL1482] - BSA and Azide free (ab313858) at 1/10000 dilution

Lane 1:

Untransfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

GFP-tagged protein transfected HEK293T whole cell extract at 30 µg

Lane 3:

RFP-tagged protein transfected HEK293T whole cell extract at 30 µg

Lane 4:

mCherry-tagged protein transfected HEK293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1482

Isotype

IgG

Carrier free

Yes

Applications

WB, ICC/IF

applications

Specificity

This antibody is specific for mCherry protein, and it does not cross-react with RFP and GFP.

style
left-column

Complementary Products

Assay Kits

AB221829

mCherry ELISA Kit

Recombinant|SimpleStep

ELISA - mCherry ELISA Kit (AB221829)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Primary Antibodies

AB185921

Anti-RFP antibody [EPR18992]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (AB185921)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information mCherry
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com