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AB167199

Anti-MCJ antibody

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(1 Publication)

Mouse Polyclonal MCJ antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DNAJC15.

View Alternative Names

DNAJD1, GIG22, HSD18, DNAJC15, DnaJ homolog subfamily C member 15, Cell growth-inhibiting gene 22 protein, Methylation-controlled J protein, MCJ

1 Images
Western blot - Anti-MCJ antibody (AB167199)
  • WB

Unknown

Western blot - Anti-MCJ antibody (AB167199)

All lanes:

Western blot - Anti-MCJ antibody (ab167199) at 1 µg/mL

Lane 1:

Lysate of 293T cells transfected with DNAJC15 at 15 µL

Lane 2:

Lysate of 293T cells (non-transfected) at 15 µL

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DNAJC15.

Q9Y5T4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCJ also known as DNAJC15 is a mitochondrial protein with an approximate mass of 17 kDa. It serves various functions via its presence in different tissues including the liver and muscle. MCJ belongs to the J-protein family and it regulates protein folding and mitochondrial function. Structurally MCJ acts as a co-chaperone and influences mitochondrial respiration by inhibiting the mitochondrial respiratory chain complex I.
Biological function summary

The role of MCJ extends beyond its mechanical actions. The protein participates in an intricate process that affects the cell's metabolism and energy production. MCJ often functions as part of a complex involving several mitochondrial proteins. It regulates mitochondrial energy production by inhibiting the activity of respiratory chain complexes leading to a direct impact on ATP synthesis and cellular metabolism.

Pathways

MCJ influences mitochondrial bioenergetics and oxidative stress pathways. It is intertwined with the mitochondrial respiratory chain pathway where it modulates the activity of complex I thereby affecting energy production and cell survival. MCJ interacts with other proteins such as NDUFA9 a component of complex I and plays a role in maintaining mitochondrial efficiency and reducing oxidative damage under stress conditions.

MCJ is associated with cancer and metabolic syndrome. Decreased expression of MCJ has been linked with various cancers due to its impact on cell survival and proliferation. In metabolic disorders alterations in MCJ expression affect energy metabolism and insulin sensitivity. Related proteins like the anti-apoptotic Bcl-2 family can further connect MCJ to these conditions illustrating the protein’s influence on disease-related mitochondrial dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.
See full target information DNAJC15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 41:1113-1121 PubMed30431104

2018

Long non‑coding RNA MALAT1 correlates with cell viability and mobility by targeting miR‑22‑3p in renal cell carcinoma via the PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Li,Zhiqiang Ma,Xiangdong Xu
View all publications
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