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AB243136

Anti-MCL1 antibody [19C4-15]

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(8 Publications)

Rat Recombinant Monoclonal MCL1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications.

View Alternative Names

BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3

1 Images
Western blot - Anti-MCL1 antibody [19C4-15] (AB243136)
  • WB

Supplier Data

Western blot - Anti-MCL1 antibody [19C4-15] (AB243136)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 10 secs.

All lanes:

Western blot - Anti-MCL1 antibody [19C4-15] (ab243136) at 1/1000 dilution

Lane 1:

SK-MEL-2 (human skin malignant melanoma) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/100000 dilution

Predicted band size: 37 kDa

false

  • Carrier free

    Anti-MCL1 antibody [19C4-15] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

19C4-15

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.31 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.31 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.
Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
See full target information MCL1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 32:1382-1396 PubMed40204951

2025

Re-appraising assays on permeabilized blood cancer cells testing venetoclax or other BH3 mimetic agents selectively targeting pro-survival BCL2 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Nan Gong,Tirta M Djajawi,Donia M Moujalled,Giovanna Pomilio,Tiffany Khong,Li-Ping Zhang,Pasquale L Fedele,Michael S Low,Mary Ann Anderson,Christopher D Riffkin,Christine A White,Ping Lan,Guillaume Lessene,Marco J Herold,Andreas Strasser,Andrew Spencer,George Grigoriadis,Andrew H Wei,Mark F van Delft,Andrew W Roberts,David C S Huang

BMC cancer 24:1350 PubMed39497108

2024

Osteosarcoma cells depend on MCL-1 for survival, and osteosarcoma metastases respond to MCL-1 antagonism plus regorafenib in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhao Ji,Michael A Harris,Lucas M Newton,Tiffany J Harris,W Douglas Fairlie,Erinna F Lee,Christine J Hawkins

Endocrine journal 71:925-933 PubMed38839345

2024

[F]FB(ePEG12)12-exendin-4 noninvasive imaging of insulinoma negative for insulin immunostaining on specimen from endoscopic ultrasonography-guided fine needle aspiration: a case report with review of literature.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Otani,Takaaki Murakami,Saeko Murakami,Ikuko Hanaoka,Hiroyuki Fujimoto,Yoichi Shimizu,Kanae Kawai Miyake,Kentaro Sakaki,Yohei Ueda,Daisuke Tanaka,Tsuyoshi Ohno,Hironori Shimizu,Naoki Uyama,Norishige Iizuka,Daisuke Yabe,Yuji Nakamoto,Nobuya Inagaki

Animal models and experimental medicine 6:245-254 PubMed37271936

2023

Anti-apoptotic protein BCL-XL as a therapeutic vulnerability in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yumin Wei,Liping Zhang,Chao Wang,Zefeng Li,Mingjie Luo,Guomin Xie,Xingjiu Yang,Mengyuan Li,Shuyue Ren,Dongbing Zhao,Ran Gao,Jia-Nan Gong

Cytotechnology 73:605-617 PubMed34349350

2021

Long non-coding RNA ZFAS1 exerts a protective role to alleviate oxygen and glucose deprivation-mediated injury in ischemic stroke cell model through targeting miR-186-5p/MCL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Shen,Lan Wang,Yuejun Xu,Hongwei Wang,Shiyi He

Frontiers in cell and developmental biology 9:655628 PubMed34109173

2021

CircRNA DOCK1 Regulates miR-409-3p/MCL1 Axis to Modulate Proliferation and Apoptosis of Human Brain Vascular Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmin Ding,Xiaolong Wang,Li Han,Zhiyu Zhao,Shuai Jia,Yuanzhao Tuo

Oncology letters 21:130 PubMed33552251

2021

Long non-coding RNA SNHG20 promotes ovarian cancer development by targeting microRNA-338-3p to regulate MCL1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Wang,Zhiying Li,Hui Li,Jiao Lu,Qi Qin

Oncology reports : PubMed33469676

2021

Hsa_circ_0008537 facilitates liver carcinogenesis by upregulating MCL1 and Snail1 expression via miR‑153‑3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Yang,Xianyong Li,Jingbo Liu,Shengjie Huang,Yaguang Weng,Jing Zhu,Daiqiong Lin,Ou Jiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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