JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB28147

Anti-MCL1 antibody

Be the first to review this product! Submit a review

|

(14 Publications)

Rabbit Polyclonal MCL1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Dog, Rabbit, Monkey, Pig, Guinea pig samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MCL1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (AB28147)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (AB28147)

ab28147 staining MCL1 in human breast cancer tissue cells by Immunohistochemistry

Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody (AB28147)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody (AB28147)

ab28147 staining MCL1 in human HeLa cells by Immunocytochemistry/ immunofluorescence.

Western blot - Anti-MCL1 antibody (AB28147)
  • WB

Unknown

Western blot - Anti-MCL1 antibody (AB28147)

All lanes:

Western blot - Anti-MCL1 antibody (ab28147) at 1/500 dilution

Lane 2:

HeLa whole cell lysate

Lane 3:

3T3 whole cell lysate

Lane 4:

PC-12 whole cell lysate

Lane 5:

MDCK whole cell lysate

Lane 6:

RK-13 whole cell lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Dog, Human, Monkey, Guinea pig, Pig, Rabbit

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MCL1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q07820

Specificity

This antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" }, "Guinea pig": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" }, "Monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" }, "Pig": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" }, "Rabbit": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p>0.1 μg/ml was sufficient for detection of Mcl1 in 20μg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.
Biological function summary

MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.

Pathways

MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.

Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
See full target information MCL1
style
left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

BMC veterinary research 21:349 PubMed40380182

2025

Overexpression of MCL-1 in canine hepatocellular carcinoma and its efficacy as a prognostic marker.

Applications

Unspecified application

Species

Unspecified reactive species

Jehun Baek,Jaeho Cho,Hun-Kyeong Shin,Wan Hee Kim

PloS one 19:e0306398 PubMed39012900

2024

Evaluation of MCL-1 as a prognostic factor in canine mammary gland tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jaeho Cho,Heaji Chung,Sungin Lee,Wan Hee Kim

Cancer chemotherapy and pharmacology 93:137-149 PubMed37921901

2023

The combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yukio Tonozuka,Hiroshi Tanaka,Kazumi Nomura,Kazuya Sakaguchi,Junpei Soeda,Yoshihide Kakimoto

Haematologica 108:797-810 PubMed36226498

2023

Inhibition of casein kinase 2 sensitizes mantle cell lymphoma to venetoclax through MCL-1 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yvonne J Thus,Martin F M De Rooij,Nathalie Swier,Roderick L Beijersbergen,Jeroen E J Guikema,Marie-José Kersten,Eric Eldering,Steven T Pals,Arnon P Kater,Marcel Spaargaren

Biological procedures online 24:16 PubMed36289539

2022

Shrimp miR-965 transfers tumoricidal mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Hyueyun Kim,Ji Ha Choi,Chang Mo Moon,Jihee Lee Kang,Minna Woo,Minsuk Kim

Liver international : official journal of the International Association for the Study of the Liver 42:2855-2870 PubMed35983950

2022

Bcl-x as prognostic marker and potential therapeutic target in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Hoffmeister-Wittmann,Andreas Mock,Federico Nichetti,Felix Korell,Christoph E Heilig,Anna-Lena Scherr,Michael Günther,Thomas Albrecht,Eblina Kelmendi,Kaiyu Xu,Luisa Nader,Annika Kessler,Nathalie Schmitt,Sarah Fritzsche,Sofia Weiler,Benjamin Sobol,Albrecht Stenzinger,Stefan Boeck,Christoph B Westphalen,Klaus Schulze-Osthoff,Jörg Trojan,Thomas Kindler,Wilko Weichert,Karsten Spiekermann,Michael Bitzer,Gunnar Folprecht,Anna L Illert,Melanie Boerries,Frederick Klauschen,Sebastian Ochsenreither,Jens Siveke,Sebastian Bauer,Hanno Glimm,Benedikt Brors,Jennifer Hüllein,Daniel Hübschmann,Sebastian Uhrig,Peter Horak,Simon Kreutzfeldt,Jesus M Banales,Christoph Springfeld,Dirk Jäger,Peter Schirmacher,Stephanie Roessler,Steffen Ormanns,Benjamin Goeppert,Stefan Fröhling,Bruno C Köhler

Oxidative medicine and cellular longevity 2022:3182931 PubMed35126811

2022

miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghong Zhu,Xuewen Yang,Jing Zhou,Long Chen,Pengfei Zuo,Lin Chen,Lan Jiang,Ting Li,Dejiang Wang,Yingyi Xu,Qiushi Li,Yi Yan

The Journal of biological chemistry 296:100276 PubMed33428943

2021

Androgen receptor splicing variant 7 (ARV7) inhibits docetaxel sensitivity by inactivating the spindle assembly checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Bingbing Yu,Yanan Liu,Haoge Luo,Jiaying Fu,Yang Li,Chen Shao

Epilepsy & behavior : E&B 113:107526 PubMed33161330

2020

Impaired postnatal development of the hippocampus of Krushinsky-Molodkina rats genetically prone to audiogenic seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey A Kulikov,Nadezhda A Dorofeeva,Alexandra A Naumova,Eugenia L Harbachova,Margarita V Glazova,Elena V Chernigovskaya

PloS one 15:e0232915 PubMed32706785

2020

Promoter choice: Who should drive the CAR in T cells?

Applications

Unspecified application

Species

Unspecified reactive species

Ali Hosseini Rad S M,Aarati Poudel,Grace Min Yi Tan,Alexander D McLellan
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB32087

Anti-MCL1 antibody [Y37]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] (AB32087)

  • 5
  • 14
Primary Antibodies

AB111574

Anti-MCL1 (phospho S159) antibody

Immunocytochemistry/ Immunofluorescence - Anti-MCL1 (phospho S159) antibody (AB111574)

  • 4
  • 1
Primary Antibodies

AB246684

Anti-MCL1 antibody

Western blot - Anti-MCL1 antibody (AB246684)

  • 0
  • 0
Primary Antibodies

AB252253

Anti-MCL1 antibody [19C4-15] - BSA and Azide free

Recombinant

Western blot - Anti-MCL1 antibody [19C4-15] - BSA and Azide free (AB252253)

  • 0
  • 0
Primary Antibodies

AB243136

Anti-MCL1 antibody [19C4-15]

Recombinant

Western blot - Anti-MCL1 antibody [19C4-15] (AB243136)

  • 0
  • 0
Primary Antibodies

AB197529

Alexa Fluor® 488 Anti-MCL1 antibody [Y37]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MCL1 antibody [Y37] (AB197529)

  • 0
  • 0
Primary Antibodies

AB186822

Anti-MCL1 antibody [Y37] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MCL1 antibody [Y37] - BSA and Azide free (AB186822)

  • 1
  • 1
Primary Antibodies

AB209289

PE Anti-MCL1 antibody [Y37]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-MCL1 antibody [Y37] (AB209289)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com