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AB122478

Anti-MCM-BP antibody

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(1 Publication)

Rabbit Polyclonal MCM-BP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCMBP aa 100-250.

View Alternative Names

C10orf119, MCMBP, Mini-chromosome maintenance complex-binding protein, MCM-BP, MCM-binding protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MCM-BP antibody (AB122478)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM-BP antibody (AB122478)

ab122478 at 4 μg/ml staining MCM-BP in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM-BP antibody (AB122478)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM-BP antibody (AB122478)

ab122478 at 1/50 dilution staining MCM-BP in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Western blot - Anti-MCM-BP antibody (AB122478)
  • WB

Unknown

Western blot - Anti-MCM-BP antibody (AB122478)

All lanes:

Western blot - Anti-MCM-BP antibody (ab122478) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 73 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MCMBP aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BTE3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MCM-BP also known as Minichromosome Maintenance Binding Protein functions as a modulator in DNA replication. This protein works by interacting with MCM complexes to regulate their activity. MCM-BP has a molecular mass of approximately 97 kDa and is expressed in several human tissues with high levels observed in proliferating cells such as those in the liver and testes. This indicates its important role in cell replication processes.
Biological function summary

The MCM-BP protein plays a role in overseeing DNA synthesis ensuring that genetic material duplicates correctly during cell division. It forms part of the DNA replication licensing complex interacting with components of the MCM complex to unload them from DNA after replication initiation. By regulating these complexes MCM-BP helps maintain the integrity and fidelity of the replication process. The protein aids in preventing genome instability which is critical for normal cellular function.

Pathways

One can understand the involvement of MCM-BP in the DNA replication pathway and the cell cycle regulation pathway. It interacts closely with proteins in the Minichromosome Maintenance (MCM) complex specifically with the helicase activity important for DNA unwinding during replication. By controlling MCM complex disassembly MCM-BP ensures the proper progression and completion of the replication process linking it to an orderly passage through the cell cycle phases.

MCM-BP has associations with certain cancers and genomic instability disorders. Its dysregulation can lead to improper DNA replication resulting in mutations and an increased risk of tumor development. For example in some cancers abnormal MCM-BP activity is observed contributing to the unchecked proliferation of cancer cells. Additionally its connection with proteins like p53 highlights its relevance in pathways that when faltered contribute to oncogenesis and other replication-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
See full target information MCMBP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 11:421-426 PubMed26893625

2016

Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Fan,Dechuan Li,Jun Qian,Yong Liu,Haiyang Feng,Dechuan Li
View all publications
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