Rabbit Polyclonal MCM-BP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCMBP aa 100-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MCMBP aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTE3
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Alternative names
C10orf119, MCMBP, Mini-chromosome maintenance complex-binding protein, MCM-BP, MCM-binding protein
Recommended products
Rabbit Polyclonal MCM-BP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCMBP aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MCMBP aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTE3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MCM-BP also known as Minichromosome Maintenance Binding Protein functions as a modulator in DNA replication. This protein works by interacting with MCM complexes to regulate their activity. MCM-BP has a molecular mass of approximately 97 kDa and is expressed in several human tissues with high levels observed in proliferating cells such as those in the liver and testes. This indicates its important role in cell replication processes.
- Biological function summary
The MCM-BP protein plays a role in overseeing DNA synthesis ensuring that genetic material duplicates correctly during cell division. It forms part of the DNA replication licensing complex interacting with components of the MCM complex to unload them from DNA after replication initiation. By regulating these complexes MCM-BP helps maintain the integrity and fidelity of the replication process. The protein aids in preventing genome instability which is critical for normal cellular function.
- Pathways
One can understand the involvement of MCM-BP in the DNA replication pathway and the cell cycle regulation pathway. It interacts closely with proteins in the Minichromosome Maintenance (MCM) complex specifically with the helicase activity important for DNA unwinding during replication. By controlling MCM complex disassembly MCM-BP ensures the proper progression and completion of the replication process linking it to an orderly passage through the cell cycle phases.
- Associated diseases and disorders
MCM-BP has associations with certain cancers and genomic instability disorders. Its dysregulation can lead to improper DNA replication resulting in mutations and an increased risk of tumor development. For example in some cancers abnormal MCM-BP activity is observed contributing to the unchecked proliferation of cancer cells. Additionally its connection with proteins like p53 highlights its relevance in pathways that when faltered contribute to oncogenesis and other replication-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM-BP antibody (ab122478)
ab122478 at 1/50 dilution staining MCM-BP in paraffin-embedded Human stomach tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-MCM-BP antibody (ab122478)
ab122478 at 4 μg/ml staining MCM-BP in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
Western blot - Anti-MCM-BP antibody (ab122478)
All lanes: Western blot - Anti-MCM-BP antibody (ab122478) at 1/250 dilution
Lane 1: RT 4 cell lysate
Lane 2: U 251 MG cell lysate
Lane 3: Human plasma lysate
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 73 kDa
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