AB124290

Anti-MCM-BP antibody

Name: Anti-MCM-BP antibody (ab124290) | Abcam
Brand: Abcam Limited
SKU: AB124290

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(2 Publications)

Rabbit Polyclonal MCM-BP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MCMBP aa 150-200.

View Alternative Names

C10orf119, MCMBP, Mini-chromosome maintenance complex-binding protein, MCM-BP, MCM-binding protein

2 Images

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Immunoprecipitation - Anti-MCM-BP antibody (AB124290)

ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2 : control IgG. Predicted band size : 73 kDa. Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-MCM-BP antibody (ab124290)

Predicted band size: 73 kDa

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Western blot - Anti-MCM-BP antibody (AB124290)

All lanes:

Western blot - Anti-MCM-BP antibody (ab124290) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 73 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MCMBP aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BTE3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab124290 was affinity purified using an epitope specific to MCM-BP immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MCM-BP also known as Minichromosome Maintenance Binding Protein functions as a modulator in DNA replication. This protein works by interacting with MCM complexes to regulate their activity. MCM-BP has a molecular mass of approximately 97 kDa and is expressed in several human tissues with high levels observed in proliferating cells such as those in the liver and testes. This indicates its important role in cell replication processes.

Biological function summary

The MCM-BP protein plays a role in overseeing DNA synthesis ensuring that genetic material duplicates correctly during cell division. It forms part of the DNA replication licensing complex interacting with components of the MCM complex to unload them from DNA after replication initiation. By regulating these complexes MCM-BP helps maintain the integrity and fidelity of the replication process. The protein aids in preventing genome instability which is critical for normal cellular function.

Pathways

One can understand the involvement of MCM-BP in the DNA replication pathway and the cell cycle regulation pathway. It interacts closely with proteins in the Minichromosome Maintenance (MCM) complex specifically with the helicase activity important for DNA unwinding during replication. By controlling MCM complex disassembly MCM-BP ensures the proper progression and completion of the replication process linking it to an orderly passage through the cell cycle phases.

MCM-BP has associations with certain cancers and genomic instability disorders. Its dysregulation can lead to improper DNA replication resulting in mutations and an increased risk of tumor development. For example in some cancers abnormal MCM-BP activity is observed contributing to the unchecked proliferation of cancer cells. Additionally its connection with proteins like p53 highlights its relevance in pathways that when faltered contribute to oncogenesis and other replication-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.

See full target information MCMBP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40498073

2025

R-2-hydroxyglutarate-mediated inhibition of KDM4A compromises telomere integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Florence Couteau,Laurence M Gagné,Karine Boulay,Philippe Rousseau,Mélissa Carbonneau,Mary McQuaid,Jyoti Sharma,Christina Sawchyn,Erlinda Fernandez,Dagmar Glatz,Rana Rizk,Marie-Eve Lalonde,Yosra Mehrjoo,Tsz Wai Chu,Gaël Moquin-Beaudry,Christian Beauséjour,Mikhail Sergeev,Santiago Costantino,Daina Avizonis,Ivan Topisirovic,Nada Jabado,Hugo Wurtele,Chantal Autexier,Frédérick A Mallette

Cancer management and research 12:7451-7463 PubMed32904436

2020

LncRNA DLX6-AS1 Promotes the Progression of Neuroblastoma by Activating STAT2 via Targeting miR-506-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Hu,Huifang Sun,Jiting Hu,Xiaomin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM-BP antibody

Immunoprecipitation - Anti-MCM-BP antibody (AB124290)

Western blot - Anti-MCM-BP antibody (AB124290)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise