AB3733

Anti-MCM10 antibody

Name: Anti-MCM10 antibody (ab3733) | Abcam
Brand: Abcam Limited
SKU: AB3733
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal MCM10 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MCM10.

View Alternative Names

PRO2249, MCM10, Protein MCM10 homolog, HsMCM10

1 Images

Supplier Data

Western blot - Anti-MCM10 antibody (AB3733)

All lanes:

Western blot - Anti-MCM10 antibody (ab3733) at 1 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 98 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MCM10. The exact immunogen used to generate this antibody is proprietary information.

Q7L590

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM10 also known as Mini-Chromosome Maintenance 10 is a component of the DNA replication machinery. This protein has an approximate molecular mass of 98 kDa. It functions mainly in the nucleus and is expressed across various cell types. MCM10 interacts closely with other replication factors to ensure DNA replication progression. Its mechanical role involves stabilizing the replication fork and binding to the replication origins.

Biological function summary

MCM10 plays an important role in the initiation and elongation phases of DNA replication. It acts as a scaffold for the assembly of other replication machinery components and facilitates the recruitment of DNA polymerase alpha. MCM10 is part of the larger pre-replication complex which includes other essential proteins such as MCM2-7 Cdc45 and GINS complex. These interactions ensure accurate DNA replication and cell cycle progression.

Pathways

MCM10 integrates into the DNA replication and repair pathways. Its function is critical in maintaining DNA integrity during the S phase of the cell cycle. In the DNA replication pathway MCM10's interaction with proteins like MCM2-7 and Cdc45 ensures the formation of replication forks. In the DNA damage response pathway it also plays a role by stabilizing the replication fork under stress conditions working alongside proteins like ATM and ATR to prevent replication stress.

MCM10 has links to cancer and genomic instability disorders. Abnormal expression levels of MCM10 can contribute to increased replication stress and genomic instability factors often seen in cancerous cells. MCM10 interacts with proteins like Cdt1 and Cdc6 whose dysregulation can lead to over-replication and cancer progression. Understanding MCM10’s role in these processes can provide insights into potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed : 24726359).

See full target information MCM10

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 11:e485 PubMed34185429

2021

Aberrant MCM10 SUMOylation induces genomic instability mediated by a genetic variant associated with survival of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Tian,Zequn Lu,Siyuan Niu,Shanshan Zhang,Pingting Ying,Lu Wang,Ming Zhang,Yimin Cai,Tianyi Dong,Ying Zhu,Rong Zhong,Zhihua Wang,Jiang Chang,Xiaoping Miao

Nucleic acids research 48:7844-7855 PubMed32652013

2020

A kinase-independent function for AURORA-A in replisome assembly during DNA replication initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Estrella Guarino Almeida,Xavier Renaudin,Ashok R Venkitaraman

International journal of oncology 55:1349-1360 PubMed31638210

2019

Minichromosome maintenance protein 10 as a marker for proliferation and prognosis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Songping Xie,Wen Yuan,Tian Xie,Muhammad Jamal,Jie Huang,Qian Yin,Hengya Song,Qiuping Zhang

Oncotarget 8:104813-104830 PubMed29285216

2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinan Ma,Liangru Fei,Meiyu Zhang,Wenzhu Zhang,Xiaofang Liu,Congcong Wang,Yuan Luo,Haiyan Zhang,Yuchen Han

Molecular medicine reports 13:4872-8 PubMed27082503

2016

Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Xiang Liao,Jin-Min Zeng,Jia-Jie Zhou,Guang-Hua Yang,Kun Ding,Xian-Jue Zhang

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

Proceedings of the National Academy of Sciences of 106:15628-32 PubMed19805216

2009

Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Sub Im,Sang-Hee Ki,Andrea Farina,Dong-Soo Jung,Jerard Hurwitz,Joon-Kyu Lee

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MCM10 antibody

Western blot - Anti-MCM10 antibody (AB3733)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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