Anti-MCM2 antibody [EPR4120] (ab108935)

Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit monoclonal antibody detecting MCM2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

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Related conjugates and formulations

ab223403
Conjugated
Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120]
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Anti-MCM2 antibody [EPR4120] - BSA and Azide free
ab223402
Conjugated
Alexa Fluor® 488 Anti-MCM2 antibody [EPR4120]
ab303155
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APC Anti-MCM2 antibody [EPR4120]
ab303156
Conjugated
HRP Anti-MCM2 antibody [EPR4120]
ab310426
Conjugated
Alexa Fluor® 594 Anti-MCM2 antibody [EPR4120]
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Alexa Fluor® 555 Anti-MCM2 antibody [EPR4120]
ab303154
Conjugated
PE Anti-MCM2 antibody [EPR4120]
ab312425
Conjugated
Alexa Fluor® 568 Anti-MCM2 antibody [EPR4120]
ab317753
Carrier free
Anti-MCM2 antibody [RM1146] - BSA and Azide free
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Anti-MCM2 antibody [RM1146]
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Alexa Fluor® 750 Anti-MCM2 antibody [EPR4120]
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Anti-MCM2 antibody
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Anti-MCM2 antibody [EPR3727]
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Images

Western blot - Anti-MCM2 antibody [EPR4120] (AB108935), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Expected	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Expected	Expected
Rat	Tested	Expected	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes For unpurified, use 1/1000 - 1/5000. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000 - 1/10000	Notes For unpurified, use 1/1000 - 1/5000. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes For unpurified, use 1/250.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/200	Notes For unpurified, use 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/200	Notes For unpurified, use 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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15 products for Alternative Product

Target data

See full target information MCM2

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (PubMed:8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).

Alternative names

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4120
Purification technique: Affinity purification Protein A
Dissociation constant: 5.8 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of MCM2?

Anti-MCM2 [EPR4120] (ab108935) specifically detects a band for MCM2 (UniProt: P49736) at a molecular weight of 102kDa.

Trusted by the scientific community

Anti-MCM2 [EPR4120] (ab108935) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR4120] also available for your convenience:
ab108935, Alexa Fluor® 488 - ab223402, Alexa Fluor® 647 - ab223403, Carrier free - ab226044, PE - ab303154, APC - ab303155, HRP - ab303156, Alkaline Phosphatase - ab308707, Alexa Fluor® 594 - ab310426, Alexa Fluor® 555 - ab311952, Alexa Fluor® 568 - ab312425, Alexa Fluor® 750 - ab320919

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

Associated diseases and disorders

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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17 product images

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
All lanes: Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution
Lane 1: MCF-7 cell lysate at 10 µg
Lane 2: Ramos cell lysate at 10 µg
Lane 3: SW480 cell lysate at 10 µg
Lane 4: Molt-4 cell lysate at 10 µg
Lane 5: Jurkat cell lysate at 10 µg
Lane 6: HeLa cell lysate at 10 µg
Predicted band size: 102 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Mouse kidney tissue lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution
All lanes: Jurkat cell lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (ab108935)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Mouse kidney tissue lysate at 10 µg
Secondary
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (ab108935)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935)
MCM2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Input) at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (+)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108935 in NIH/3T3 whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 130 kDa
Exposure time: 3s
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935)
MCM2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate (Input) at 10 µg
Lane 2: C6 (rat glial tumor glial cell) whole cell lysate (+)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108935 in C6 whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 130 kDa
Exposure time: 3s