Anti-MCM2 antibody [EPR4120]
- RabMAb
- Recombinant
- What is this?
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(23 Publications)
Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit monoclonal antibody detecting MCM2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
View Alternative Names
BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28
- IP
Lab
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (AB108935)
MCM2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution
Lane 1:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Input) at 10 µg
Lane 2:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (+)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 130 kDa
false
Exposure time: 3s
- IP
Lab
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (AB108935)
MCM2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/30 dilution
Lane 1:
C6 (rat glial tumor glial cell) whole cell lysate (Input) at 10 µg
Lane 2:
C6 (rat glial tumor glial cell) whole cell lysate (+)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 130 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
Mouse kidney tissue lysate at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
false
- WB
Lab
Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution
Lane 1:
HeLa cell lysate at 10 µg
Lane 2:
Mouse kidney tissue lysate at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
false
- WB
Unknown
Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)
All lanes:
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution
Lane 1:
MCF-7 cell lysate at 10 µg
Lane 2:
Ramos cell lysate at 10 µg
Lane 3:
SW480 cell lysate at 10 µg
Lane 4:
Molt-4 cell lysate at 10 µg
Lane 5:
Jurkat cell lysate at 10 µg
Lane 6:
HeLa cell lysate at 10 µg
Predicted band size: 102 kDa
false
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)
Unpurified ab108935 at a 1/100 dilution staining MCM2 in HeLa cells.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395 at a 1/250 dilution.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] (AB108935)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395 at a 1/250 dilution.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (AB108935)
Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0.
- WB
Lab
Western blot - Anti-MCM2 antibody [EPR4120] (AB108935)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution
All lanes:
Jurkat cell lysate at 10 µg
Secondary
All lanes:
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 125 kDa
false
- OI-RD Scanning
Unknown
OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (AB108935)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Related conjugates and formulations (15)
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120]
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Anti-MCM2 antibody [EPR4120] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-MCM2 antibody [EPR4120]
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660 APC
APC Anti-MCM2 antibody [EPR4120]
-
HRP Anti-MCM2 antibody [EPR4120]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-MCM2 antibody [EPR4120]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-MCM2 antibody [EPR4120]
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578 PE
PE Anti-MCM2 antibody [EPR4120]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-MCM2 antibody [EPR4120]
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Anti-MCM2 antibody [RM1146] - BSA and Azide free
-
Anti-MCM2 antibody [RM1146]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-MCM2 antibody [EPR4120]
-
Anti-MCM2 antibody
-
Anti-MCM2 antibody [EPR3727]
-
Anti-MCM2 antibody [SP85]
Reactivity data
Product details
What is this antibody validated in?
Anti-MCM2 antibody [EPR4120] (ab108935) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of MCM2?
Anti-MCM2 [EPR4120] (ab108935) specifically detects a band for MCM2 (UniProt: P49736) at a molecular weight of 102kDa.
Trusted by the scientific community
Anti-MCM2 [EPR4120] (ab108935) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR4120] also available for your convenience: ab108935, Alexa Fluor® 488 - ab223402, Alexa Fluor® 647 - ab223403, Carrier free - ab226044, PE - ab303154, APC - ab303155, HRP - ab303156, Alkaline Phosphatase - ab308707, Alexa Fluor® 594 - ab310426, Alexa Fluor® 555 - ab311952, Alexa Fluor® 568 - ab312425, Alexa Fluor® 750 - ab320919
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.
Pathways
MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (23)
Recent publications for all applications. Explore the full list and refine your search
BMC pulmonary medicine 23:507 PubMed38093266
2023
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Stem cells international 2023:2826815 PubMed37964983
2023
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Journal of gynecologic oncology 34:e2 PubMed36245223
2022
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Frontiers in oncology 12:1000925 PubMed36248994
2022
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Disease markers 2022:6967573 PubMed36193504
2022
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eLife 11: PubMed36082941
2022
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PloS one 17:e0270880 PubMed35857794
2022
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Journal of oncology 2022:8326940 PubMed35874635
2022
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Frontiers in bioscience (Landmark edition) 27:25 PubMed35090330
2022
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Biochimica et biophysica acta. Molecular basis of disease 1867:166237 PubMed34339838
2021
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Product promise
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