Anti-MCM2 antibody [EPR4120] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

• IP

Lab

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

MCM2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/30 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate (Input) at 10 µg

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate (+)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 130 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

MCM2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab108935 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108935 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/30 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Input) at 10 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (+)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 130 kDa

false

Exposure time: 3s

• WB

Unknown

Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/10000 dilution

Lane 1:

MCF-7 cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

Molt-4 cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Predicted band size: 102 kDa

false

• WB

Lab

Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

• WB

Lab

Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/8500 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

• WB

Lab

Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

This data was developed using ab108935, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MCM2 antibody [EPR4120] (<a href='/en-us/products/primary-antibodies/mcm2-antibody-epr4120-ab108935'>ab108935</a>) at 1/8500 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa

Observed band size: 125 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (AB226044)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.