Anti-MCM2 antibody [SP85]
- BOND RX™ Validated
- RabMAb
- Recombinant
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(2 Publications)
Rabbit Recombinant Monoclonal MCM2 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
View Alternative Names
BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28
- IP
Lab
Immunoprecipitation - Anti-MCM2 antibody [SP85] (AB95361)
MCM2 was immunoprecipitated from 0.35 mg Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab95361 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab95361 at 1/1000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-MCM2 antibody [SP85] (ab95361) at 1/1000 dilution
Lane 1:
Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate (Input) at 10 µg
Lane 2:
Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate (+)
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab95361 in Hela whole cell lysate (-)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 37s
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [SP85] (AB95361)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MCM2 with ab95361 at 1/100 (0.06 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Nuclear staining on rat colon. The section was incubated with ab95361 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [SP85] (AB95361)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MCM2 with ab95361 at 1/100 (0.06 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Nuclear staining on mouse colon. The section was incubated with ab95361 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins
- IP
Lab
Immunoprecipitation - Anti-MCM2 antibody [SP85] (AB95361)
MCM2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab95361 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab95361 at 1/1000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-MCM2 antibody [SP85] (ab95361) at 1/1000 dilution
Lane 1:
C6 (rat glial tumor glial cell) whole cell lysate (Input) at 10 µg
Lane 2:
C6 (rat glial tumor glial cell) whole cell lysate (+)
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab95361 in C6 whole cell lysate (-)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
- IP
Lab
Immunoprecipitation - Anti-MCM2 antibody [SP85] (AB95361)
MCM2 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab95361 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab95361 at 1/1000 dilution.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-MCM2 antibody [SP85] (ab95361) at 1/1000 dilution
Lane 1:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (Input) at 10 µg
Lane 2:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (+)
Lane 3:
Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab95361 in NIH/3T3 whole cell lysate (-)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
false
Exposure time: 103s
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [SP85] (AB95361)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling MCM2 with purified ab95361 at 1/50 (2.5 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- WB
Unknown
Western blot - Anti-MCM2 antibody [SP85] (AB95361)
All lanes:
Western blot - Anti-MCM2 antibody [SP85] (ab95361) at 1/100 dilution
All lanes:
lysate prepared from HeLa cells
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [SP85] (AB95361)
ab95361, at 1/100 dilution, staining MCM2 in formalin-fixed, paraffin-embedded Human rectal carcinoma tissue by Immunohistochemistry.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [SP85] (AB95361)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling MCM2 with purified ab95361 at 1/50 (2.5 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [SP85] (AB95361)
Flow cytometry analysis of C6 (rat glial tumor glial cell) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [SP85] (AB95361)
Flow cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [SP85] (AB95361)
Flow cytometric analysis using rabbit anti-MCM2 (SP85) antibody ab95361(1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [SP85] (AB95361)
ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human prostate tissue by Immunohistochemistry.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [SP85] (AB95361)
ab95361,at 1/100 dilution,staining MCM2 in formalin-fixed,paraffin-embedded Human pancreas adenocarcinoma tissue by Immunohistochemistry.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-MCM2 antibody [SP85] (AB95361)
Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling MCM2 with purified ab95361 at 1/200 dilution (0.615 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
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Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.
Pathways
MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.
Product protocols
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Target data
Publications (2)
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Molecular cell 82:463-478.e11 PubMed34741808
2021
Applications
Unspecified application
Species
Unspecified reactive species
Glia 68:2361-2376 PubMed32469469
2020
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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