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Rabbit Recombinant Monoclonal MCM2 phospho S108 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 6 publications.

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Images

Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271), expandable thumbnail
  • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271), expandable thumbnail
  • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271), expandable thumbnail
  • Flow Cytometry - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Expected
Tested
Tested
Not recommended
Tested
Mouse
Tested
Expected
Tested
Not recommended
Expected
Rat
Expected
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (PubMed:8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).

Alternative names

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

Recommended products

Rabbit Recombinant Monoclonal MCM2 phospho S108 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4121
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

Associated diseases and disorders

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    MCM2 (phospho S108) was immunoprecipitated from 0.35 mg RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab109271 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109271 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate, 10µg

    Lane 2: ab109271 IP in RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109271in RAW 264.7 whole cell lysate.

    All lanes: Immunoprecipitation - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/1000 dilution

    All lanes: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 125 kDa

    Exposure time: 1s

  • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    All lanes: Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/10000 dilution

    Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 2: C6 whole cell lysate, Then the membrane was incubated with alkaline phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 125 kDa

    Exposure time: 5s

  • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    All lanes: Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/10000 dilution

    Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg with 5% NFDM/TBST

    Lane 2: RAW 264.7 whole cell lysate, Then the membrane was incubated with alkaline phosphatase at 15 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 125 kDa

    Exposure time: 5s

  • Flow Cytometry - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Flow Cytometry - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell) cells labelling MCM2 (phospho S108) with ab109271 at 1/80 dilution (1ug)/red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling MCM2 (phospho S108) with ab109271 at 1/2000 (0.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in HeLa cells. The signal decreased after alkaline phosphatase treatment at 37℃ for 2h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271), expandable thumbnail

    Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)

    All lanes: Western blot - Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg with 5% NFDM/TBST

    Lane 2: HeLa whole cell lysate, Then the membrane was incubated with alkaline phosphatase at 15 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 125 kDa

    Exposure time: 1s

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Product protocols

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