AB133243

Anti-MCM2 (phospho S40) antibody [EPR4170(2)]

Name: Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (ab133243) | Abcam
Brand: Abcam Limited
SKU: AB133243
Price: 260 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(21 Publications)

Rabbit Recombinant Monoclonal MCM2 phospho S40 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 21 publications.

View Alternative Names

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MCM2 (phospho S40) with ab133243 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

All lanes:

Western blot - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (ab133243) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate alkaline phosphatase treated at 10 µg

Predicted band size: 102 kDa

false

Carrier free

Anti-MCM2 (phospho S40) antibody [EPR4170(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4170(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2 pS40

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5706 PubMed40593507

2025

DNA polymerase α/primase extraction from chromatin by VCP/p97 restricts ATR activation during unperturbed DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Rodríguez-Acebes,Rodrigo Martín-Rufo,Alicia Gómez-Moya,Scott B Churcher,Alejandro Fernández-Llorente,Guillermo de la Vega-Barranco,Alejandra Perona,Pilar Oroz,Elena Martín-Doncel,Luis Ignacio Toledo,Juan Méndez,Emilio Lecona

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403782 PubMed39412086

2024

CDC7 Inhibition Potentiates Antitumor Efficacy of PARP Inhibitor in Advanced Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shini Liu,Peng Deng,Zhaoliang Yu,Jing Han Hong,Jiuping Gao,Yulin Huang,Rong Xiao,Jiaxin Yin,Xian Zeng,Yichen Sun,Peili Wang,Ruizi Geng,Jason Yongsheng Chan,Peiyong Guan,Qiang Yu,Bin-Tean Teh,Qingping Jiang,Xiaojun Xia,Ying Xiong,Jianfeng Chen,Yongliang Huo,Jing Tan

The EMBO journal 43:1301-1324 PubMed38467834

2024

ATR limits Rad18-mediated PCNA monoubiquitination to preserve replication fork and telomerase-independent telomere stability.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Chen,Chen Pan,Jun Huang,Ting Liu

Nature communications 14:7490 PubMed37980406

2023

CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Yamamori Morita,Jie Yu,Yukie Kashima,Ryo Kamata,Gaku Yamamoto,Tatsunori Minamide,Chiaki Mashima,Miyuki Yoshiya,Yuta Sakae,Toyohiro Yamauchi,Yumi Hakozaki,Shun-Ichiro Kageyama,Akito Nakamura,Eric Lightcap,Kosuke Tanaka,Huifeng Niu,Karuppiah Kannan,Akihiro Ohashi

EMBO reports 24:e57585 PubMed37965896

2023

The nucleolar protein GNL3 prevents resection of stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Rana Lebdy,Marine Canut,Julie Patouillard,Jean-Charles Cadoret,Anne Letessier,Josiane Ammar,Jihane Basbous,Serge Urbach,Benoit Miotto,Angelos Constantinou,Raghida Abou Merhi,Cyril Ribeyre

Nature communications 14:3618 PubMed37336885

2023

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Demis Menolfi,Brian J Lee,Hanwen Zhang,Wenxia Jiang,Nicole E Bowen,Yunyue Wang,Junfei Zhao,Antony Holmes,Steven Gershik,Raul Rabadan,Baek Kim,Shan Zha

Nature communications 14:1353 PubMed36906648

2023

Short-term molecular consequences of chromosome mis-segregation for genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenza Garribba,Giuseppina De Feudis,Valentino Martis,Martina Galli,Marie Dumont,Yonatan Eliezer,René Wardenaar,Marica Rosaria Ippolito,Divya Ramalingam Iyer,Andréa E Tijhuis,Diana C J Spierings,Michael Schubert,Silvia Taglietti,Chiara Soriani,Simon Gemble,Renata Basto,Nick Rhind,Floris Foijer,Uri Ben-David,Daniele Fachinetti,Ylli Doksani,Stefano Santaguida

iScience 25:104752 PubMed35942091

2022

Co-targeting of specific epigenetic regulators in combination with CDC7 potently inhibit melanoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Chava,Suresh Bugide,Parmanand Malvi,Romi Gupta

Nature 605:357-365 PubMed35508654

2022

CDC7-independent G1/S transition revealed by targeted protein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jan M Suski,Nalin Ratnayeke,Marcin Braun,Tian Zhang,Vladislav Strmiska,Wojciech Michowski,Geylani Can,Antoine Simoneau,Konrad Snioch,Mikolaj Cup,Caitlin M Sullivan,Xiaoji Wu,Joanna Nowacka,Timothy B Branigan,Lindsey R Pack,James A DeCaprio,Yan Geng,Lee Zou,Steven P Gygi,Johannes C Walter,Tobias Meyer,Piotr Sicinski

Genome medicine 13:166 PubMed34663432

2021

Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchen Guo,Jun Wang,Bente Benedict,Chen Yang,Frank van Gemert,Xuhui Ma,Dongmei Gao,Hui Wang,Shu Zhang,Cor Lieftink,Roderick L Beijersbergen,Hein Te Riele,Xiaohang Qiao,Qiang Gao,Chong Sun,Wenxin Qin,René Bernards,Cun Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM2 (phospho S40) antibody [EPR4170(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

Western blot - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise