AB70371

Anti-MCM2 (phospho S40 + S41) antibody

Name: Anti-MCM2 (phospho S40 + S41) antibody (ab70371) | Abcam
Brand: Abcam Limited
SKU: AB70371
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal MCM2 phospho S40 + S41 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MCM2 pS40 + S41.

View Alternative Names

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

3 Images

Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

ab70371 at 0.1μg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x10⁶ jurkat cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling MCM2 (phospho S40 + S41) with ab70371 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection : Chemiluminescence with exposure times of 30 seconds for upper and lower panel.

All lanes:

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (ab70371) at 0.1 µg/mL

Lane 1:

Whole cell lysate from asynchronous 293T cells at 50 µg

Lane 2:

Whole cell lysate from asynchronous 293T cells treated with a phosphatase at 50 µg

Predicted band size: 102 kDa

Observed band size: 117 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human MCM2 pS40 + S41. The exact immunogen used to generate this antibody is proprietary information.

P49736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "0.1 μg per 1 X 106 cells in a 150 μl volume/<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2 pS40 + S41

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed36009559

2022

Dbf4-Cdc7 (DDK) Inhibitor PHA-767491 Displays Potent Anti-Proliferative Effects via Crosstalk with the CDK2-RB-E2F Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tekle Pauzaite,James Tollitt,Betul Sopaci,Louise Caprani,Olivia Iwanowytsch,Urvi Thacker,John G Hardy,Sarah L Allinson,Nikki A Copeland

Cell death discovery 8:85 PubMed35220396

2022

CDC7 kinase (DDK) inhibition disrupts DNA replication leading to mitotic catastrophe in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey C Martin,Jennie R Sims,Ajay Gupta,Tamara J Hagoel,Lingqiu Gao,Miranda L Lynch,Anna Woloszynska,Thomas Melendy,Jeremy F Kane,Joseph Kuechle,Joyce E Ohm

Journal of experimental & clinical cancer research : CR 40:82 PubMed33648524

2021

Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention.

Applications

Unspecified application

Species

Unspecified reactive species

Esmee Koedoot,Eline van Steijn,Marjolein Vermeer,Román González-Prieto,Alfred C O Vertegaal,John W M Martens,Sylvia E Le Dévédec,Bob van de Water

Breast cancer research : BCR 21:77 PubMed31262335

2019

A kinase inhibitor screen identifies a dual cdc7/CDK9 inhibitor to sensitise triple-negative breast cancer to EGFR-targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ronan P McLaughlin,Jichao He,Vera E van der Noord,Jevin Redel,John A Foekens,John W M Martens,Marcel Smid,Yinghui Zhang,Bob van de Water

Cancer cell international 16:88 PubMed27891063

2016

Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness.

Applications

Species

Human

Zubeyde Erbayraktar,Begum Alural,Resat Serhat Erbayraktar,Erdogan Pekcan Erkan

View all publications

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Product promise

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Anti-MCM2 (phospho S40 + S41) antibody

Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise