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AB272877

Anti-MCM3 antibody

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(2 Publications)

Rabbit Polyclonal MCM3 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM3.

View Alternative Names

DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody (AB272877)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody (AB272877)

4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCM3 using ab272877 at 1/500 dilution in ICC/IF.

Red : alpha Tubulin, a cytoskeleton marker.

Blue : Hoechst 33342 staining.

Immunoprecipitation - Anti-MCM3 antibody (AB272877)
  • IP

Unknown

Immunoprecipitation - Anti-MCM3 antibody (AB272877)

Samples : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
A. 40 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM3 protein by 4 μg of ab272877

7.5 % SDS-PAGE
The immunoprecipitated MCM3 protein was detected by ab272877 diluted at 1 : 1000, followed by anti-rabbit IgG.

All lanes:

Immunoprecipitation - Anti-MCM3 antibody (ab272877)

Predicted band size: 91 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (AB272877)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (AB272877)

Paraffin-embedded mouse colon tissue stained for MCM3 using ab272877 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.

Western blot - Anti-MCM3 antibody (AB272877)
  • WB

Supplier Data

Western blot - Anti-MCM3 antibody (AB272877)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM3 antibody (ab272877) at 1/1000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 91 kDa

false

Western blot - Anti-MCM3 antibody (AB272877)
  • WB

Supplier Data

Western blot - Anti-MCM3 antibody (AB272877)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM3 antibody (ab272877) at 1/500 dilution

All lanes:

Mouse colon tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 91 kDa

false

Western blot - Anti-MCM3 antibody (AB272877)
  • WB

Supplier Data

Western blot - Anti-MCM3 antibody (AB272877)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM3 antibody (ab272877) at 1/500 dilution

Lane 1:

Neuro2a whole cell extract at 30 µg

Lane 2:

NIH-3T3 whole cell extract at 30 µg

Lane 3:

Raw264.7 whole cell extract at 30 µg

Lane 4:

C2C12 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCM3. The exact immunogen used to generate this antibody is proprietary information.

P25205

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Minichromosome Maintenance Complex Component 3 also called MCM3 plays an important role in DNA replication. It is part of the MCM complex which acts as a helicase that unwinds DNA as part of its function. MCM3 has a molecular mass of approximately 100 kDa and is expressed across various tissues reflecting its fundamental role in cell cycle regulation. Researchers have identified MCM3’s presence not only in proliferating cells but also in areas of high DNA replication demand such as during embryonic development.
Biological function summary

The MCM3 protein is critical in establishing the prereplication complex which initiates DNA synthesis. It forms a heterohexamer along with other MCM proteins (MCM2 MCM4 MCM5 MCM6 and MCM7) constituting the core of the replication licensing system. By controlling the replication fork progression MCM3 ensures precise replication and genome stability. The complex’s activity is essential for the successful transition from the G1 phase to the S phase of the cell cycle.

Pathways

MCM3 is an important component of the cell cycle control pathway and DNA damage response pathway. It interacts with other key proteins such as CDC6 and CDT1 which regulate its loading onto chromatin. MCM3's helicase activity is an integral part of the replication machinery ensuring that DNA replication proceeds smoothly and accurately. By coordinating with proteins like Cyclin E MCM3 helps maintain the fidelity and timing of DNA replication safeguarding the genetic material.

Irregular expression or function of MCM3 has been linked to cancer and genomic instability disorders. Cancer cells often exhibit high levels of MCM3 which correlates with increased proliferation rates and aggressive tumor growth. Connections between MCM3 and the p53 protein highlight its potential role in tumorigenesis as p53 mutations often result in loss of genome integrity which MCM3 helps to safeguard. As such MCM3 serves not only as a marker for cancer diagnosis but also potentially as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (Probable).
See full target information MCM3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113273 PubMed37858471

2023

mA demethylase FTO stabilizes LINK-A to exert oncogenic roles via MCM3-mediated cell-cycle progression and HIF-1α activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yabing Nan,Shi Liu,Qingyu Luo,Xiaowei Wu,Pengfei Zhao,Wan Chang,Ruixiang Zhang,Yin Li,Zhihua Liu

Helicobacter 26:e12849 PubMed34490965

2021

Upregulation of oncogene Activin A receptor type I by Helicobacter pylori infection promotes gastric intestinal metaplasia via regulating CDX2.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yan Chen,Yi Hu,Xin-Bo Xu,Yan-An Zhou,Nian-Shuang Li,Cong He,Chuan Xie,Nong-Hua Lu,Yin Zhu
View all publications
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