Anti-MCM3 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody (AB4460)

ICC/IF image of ab4460 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4460, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (AB4460)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MCM3 with ab4460 at 1/1000 (1µg/ml). Detection : DAB.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody (AB4460)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling MCM3 with ab4460 at 1/1000 (1μg/ml). Detection : DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-MCM3 antibody (AB4460)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lanes 1 and 2 : ab4460 used for IP at 6 μg per reaction, two lots tested.

Lane 3 Control IgG.

For blotting immunoprecipitated MCM3, ab4460 was used at 0.04 μg/ml.

All lanes:

Immunoprecipitation - Anti-MCM3 antibody (ab4460)

Predicted band size: 91 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-MCM3 antibody (AB4460)

Whole cell lysate (10 μg) from HeLa, Jurkat, HEK-293T, and TCMK-1 cells prepared using NETN lysis buffer.

ab4460 used for WB at 0.04 μg/ml.

All lanes:

Western blot - Anti-MCM3 antibody (ab4460)

Predicted band size: 91 kDa

false

Exposure time: 3s

• WB

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Western blot - Anti-MCM3 antibody (AB4460)

WB using ab4460. Western blot analysis of ab4460 on HeLa OHIO extract.

All lanes:

Western blot - Anti-MCM3 antibody (ab4460)

Predicted band size: 91 kDa

false

• WB

Unknown

Western blot - Anti-MCM3 antibody (AB4460)

HeLa cell RIPA extract (50 μg) incubated with ab4460 used at 0.2 μg/ml for 1 hour. HeLa cell RIPA extract (50 µg) incubated with ab4460 used at 0.2 µg/ml for 1 hour.

All lanes:

Western blot - Anti-MCM3 antibody (ab4460)

Predicted band size: 91 kDa

false