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AB317372

Anti-MCM3 antibody [S04-6B9]

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Rabbit Monoclonal MCM3 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Synthetic Peptide within Human MCM3.

View Alternative Names

DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 Antibody [S04-6B9] (AB317372)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 Antibody [S04-6B9] (AB317372)

Immunohistochemistry of MCM3 in paraffin-embedded human tonsil using ab317372 at dilution 1/1.

Immunocytochemistry/ Immunofluorescence - Anti-MCM3 Antibody [S04-6B9] (AB317372)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCM3 Antibody [S04-6B9] (AB317372)

Immunofluorescence of MCM3 (green) in HeLa cells using ab317372 at dilution 1/200, and DAPI (blue).

Western blot - Anti-MCM3 Antibody [S04-6B9] (AB317372)
  • WB

Supplier Data

Western blot - Anti-MCM3 Antibody [S04-6B9] (AB317372)

All lanes:

Western blot - Anti-MCM3 Antibody [S04-6B9] (ab317372) at 1/1000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

C6 cell lysate

Lane 3:

HeLa cell lysate

Predicted band size: 91 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S04-6B9

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MCM3.

P25205

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Minichromosome Maintenance Complex Component 3 also called MCM3 plays an important role in DNA replication. It is part of the MCM complex which acts as a helicase that unwinds DNA as part of its function. MCM3 has a molecular mass of approximately 100 kDa and is expressed across various tissues reflecting its fundamental role in cell cycle regulation. Researchers have identified MCM3’s presence not only in proliferating cells but also in areas of high DNA replication demand such as during embryonic development.
Biological function summary

The MCM3 protein is critical in establishing the prereplication complex which initiates DNA synthesis. It forms a heterohexamer along with other MCM proteins (MCM2 MCM4 MCM5 MCM6 and MCM7) constituting the core of the replication licensing system. By controlling the replication fork progression MCM3 ensures precise replication and genome stability. The complex’s activity is essential for the successful transition from the G1 phase to the S phase of the cell cycle.

Pathways

MCM3 is an important component of the cell cycle control pathway and DNA damage response pathway. It interacts with other key proteins such as CDC6 and CDT1 which regulate its loading onto chromatin. MCM3's helicase activity is an integral part of the replication machinery ensuring that DNA replication proceeds smoothly and accurately. By coordinating with proteins like Cyclin E MCM3 helps maintain the fidelity and timing of DNA replication safeguarding the genetic material.

Irregular expression or function of MCM3 has been linked to cancer and genomic instability disorders. Cancer cells often exhibit high levels of MCM3 which correlates with increased proliferation rates and aggressive tumor growth. Connections between MCM3 and the p53 protein highlight its potential role in tumorigenesis as p53 mutations often result in loss of genome integrity which MCM3 helps to safeguard. As such MCM3 serves not only as a marker for cancer diagnosis but also potentially as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (Probable).
See full target information MCM3
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Product promise

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