Rabbit Polyclonal MCM4 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCM4 aa 550-850.
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- Signal transduction and targeted therapy 7:1022022LSD1 is required for euchromatic origin firing and replication timing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Wang,Yunchao Huang,Edith Cheng,Xinhua Liu,Yu Zhang,Jianguo Yang,Jordan T F Young,Grant W Brown,Xiaohan Yang,Yongfeng ShangPubMed 35414135
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MCM4 aa 550-850. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
Reactivity data
Select an application| IHC-P | IP | ChIP | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Assay dependent |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914).
Alternative names
CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21
Recommended products
Rabbit Polyclonal MCM4 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCM4 aa 550-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MCM4 aa 550-850. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.
- Biological function summary
MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.
- Pathways
Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.
- Associated diseases and disorders
Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-MCM4 antibody (ab154315)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-MCM4 antibody (ab154315) at 1/5000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
Predicted band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab154315)
Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling MCM4 using ab154315 at 1/100 dilution.
Immunoprecipitation - Anti-MCM4 antibody (ab154315)
MCM4 was immunoprecipitated from 40 μg of Jurkat whole cell lysate with ab154315 at 1/500 dilution. 7.5 % SDS-PAGE. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: Jurkat lysate 40 μg (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody.All lanes: Immunoprecipitation - Anti-MCM4 antibody (ab154315)
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab154315)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MCM4 using ab154315 at 1/500 a dilution. Right panel costained with Hoechst 33342.
Western blot - Anti-MCM4 antibody (ab154315)
7.5% SDS-PAGE
All lanes: Western blot - Anti-MCM4 antibody (ab154315) at 1/1000 dilution
Lane 1: Neuro2A whole cell extract at 30 µg
Lane 2: C8D30 whole cell extract at 30 µg
Lane 3: NIH/3T3 whole cell extract at 30 µg
Lane 4: Raw264.7 whole cell extract at 30 µg
Lane 5: C2C12 whole cell extract at 30 µg
Predicted band size: 97 kDa
ChIP - Anti-MCM4 antibody (ab154315)
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
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