Goat Polyclonal MCM4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4.
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- The journal of obstetrics and gynaecology research 47:184-1972020MiR-95-5p involves in the migration and invasion of trophoblast cells by targeting low density lipoprotein receptor-related protein 6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuijie Ni,Xiao Wang,Hongmei Qu,Xueli Gao,Xiaoyan YuPubMed 32924237
- Molecular medicine reports 22:2810-28162020Downregulated miR‑130a enhances the sensitivity of acute myeloid leukemia cells to Adriamycin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Liu et. al.PubMed 32945422
- Molecular medicine (Cambridge, Mass.) 26:492020Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3β/β-catenin and TGF-β1/Smad2/3 signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQun Lv et. al.PubMed 32448163
- International journal of molecular medicine 46:211-2232020LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang et. al.PubMed 32319541
- Cytotechnology 71:1155-11652019MiR-214-3p regulates the viability, invasion, migration and EMT of TNBC cells by targeting ST6GAL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Tao et. al.PubMed 31705333
- The Journal of cell biology 178:257-682007An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.Applications:WBReactive species:Chicken, HumanHelfrid Hochegger,Donniphat Dejsuphong,Eiichiro Sonoda,Alihossein Saberi,Eeson Rajendra,Jane Kirk,Tim Hunt,Shunichi TakedaPubMed 17635936
- The Journal of biological chemistry 282:208-152006Cdc7 is an active kinase in human cancer cells undergoing replication stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPierluigi Tenca,Deborah Brotherton,Alessia Montagnoli,Sonia Rainoldi,Clara Albanese,Corrado SantocanalePubMed 17062569
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MCM4. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914).
Alternative names
CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21
Recommended products
Goat Polyclonal MCM4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MCM4. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.
- Biological function summary
MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.
- Pathways
Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.
- Associated diseases and disorders
Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-MCM4 antibody (ab3728)
All lanes: Western blot - Anti-MCM4 antibody (ab3728) at 1/10000 dilution
Lane 1: HeLa Whole cell lysate at 50 µg
Lane 2: HEK293T Whole cell lysate at 50 µg
Lane 3: Jurkat Whole cell lysate at 50 µg
Predicted band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1μg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1μg/ml). Detection: DAB.
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