AB3728

Anti-MCM4 antibody

Name: Anti-MCM4 antibody (ab3728) | Abcam
Brand: Abcam Limited
SKU: AB3728
Price: 535 USD
Availability: InStock

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(7 Publications)

Goat Polyclonal MCM4 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4.

View Alternative Names

CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1μg/ml). Detection : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1μg/ml). Detection : DAB.

Supplier Data

Western blot - Anti-MCM4 antibody (AB3728)

All lanes:

Western blot - Anti-MCM4 antibody (ab3728) at 1/10000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

Jurkat Whole cell lysate at 50 µg

Predicted band size: 97 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MCM4. The exact immunogen used to generate this antibody is proprietary information.

P33991

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.

Biological function summary

MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.

Pathways

Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.

Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 9305914).

See full target information MCM4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The journal of obstetrics and gynaecology research 47:184-197 PubMed32924237

2020

MiR-95-5p involves in the migration and invasion of trophoblast cells by targeting low density lipoprotein receptor-related protein 6.

Applications

Unspecified application

Species

Unspecified reactive species

Huijie Ni,Xiao Wang,Hongmei Qu,Xueli Gao,Xiaoyan Yu

Molecular medicine reports 22:2810-2816 PubMed32945422

2020

Downregulated miR‑130a enhances the sensitivity of acute myeloid leukemia cells to Adriamycin.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Liu,Min Liu,Jiangzhao Zhang,Yan Liang

Molecular medicine (Cambridge, Mass.) 26:49 PubMed32448163

2020

Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3β/β-catenin and TGF-β1/Smad2/3 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Lv,Jianjun Wang,Changqing Xu,Xuqing Huang,Zhaoyang Ruan,Yifan Dai

International journal of molecular medicine 46:211-223 PubMed32319541

2020

LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR‑346.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Huihui Li,Mingbao Li,Wenxia Zhang,Zhou Yang,Shuquan Zhang

Cytotechnology 71:1155-1165 PubMed31705333

2019

MiR-214-3p regulates the viability, invasion, migration and EMT of TNBC cells by targeting ST6GAL1.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Tao,Zhijing Zhao,Junfeng Ma,Liying Dong,Ying Liang,Siqi Li,Ying Mao,Yan Li,Yi Zhang

The Journal of cell biology 178:257-68 PubMed17635936

2007

An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.

Applications

WB, WB

Species

Chicken, Human

Helfrid Hochegger,Donniphat Dejsuphong,Eiichiro Sonoda,Alihossein Saberi,Eeson Rajendra,Jane Kirk,Tim Hunt,Shunichi Takeda

The Journal of biological chemistry 282:208-15 PubMed17062569

2006

Cdc7 is an active kinase in human cancer cells undergoing replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Pierluigi Tenca,Deborah Brotherton,Alessia Montagnoli,Sonia Rainoldi,Clara Albanese,Corrado Santocanale

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

Western blot - Anti-MCM4 antibody (AB3728)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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